@inbook{11456,
  abstract     = {The proteomes of specialized structures, and the interactomes of proteins of interest, provide entry points to elucidate the functions of molecular machines. Here, we review a proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes. Although the focus is on C. elegans neurons, the method is applicable regardless of cell type. We describe detailed extraction procedures that solubilize the bulk of C. elegans proteins and highlight the importance of tagging endogenous genes, to ensure physiological expression levels. We review issues associated with non-specific background noise and the importance of appropriate controls. As proof of principle, we review our analysis of the interactome of a presynaptic active zone protein, ELKS-1. Our aim is to provide a detailed protocol for TurboID-based proximity labeling in C. elegans and to highlight its potential and its limitations to characterize protein complexes and subcellular compartments in this animal.},
  author       = {Artan, Murat and de Bono, Mario},
  booktitle    = {Behavioral Neurogenetics},
  editor       = {Yamamoto, Daisuke},
  isbn         = {9781071623206},
  issn         = {1940-6045},
  pages        = {277--294},
  publisher    = {Springer Nature},
  title        = {{Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling}},
  doi          = {10.1007/978-1-0716-2321-3_15},
  volume       = {181},
  year         = {2022},
}