article published yes Christoph Gerle author Jun-ichi Kishikawa author Tomoko Yamaguchi author Atsuko Nakanishi author Mehmet Orkun Çoruh author d25163e5-8d53-11eb-a251-e6dd8ea1b8ef0000-0002-3219-2022 Fumiaki Makino author Tomoko Miyata author Akihiro Kawamoto author Ken Yokoyama author Keiichi Namba author Genji Kurisu author Takayuki Kato author LeSa department Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica. https://research-explorer.ista.ac.at/download/11648/12498/2022_Microscopy_Gerle.pdf application/pdfno Oxford University Press2022 eng RadiologyNuclear Medicine and imagingInstrumentationStructural Biology 2050-5698 2050-5701 35861182 00083795090000110.1093/jmicro/dfac037 715249-261 Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. 2022. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 71(5), 249–261. C. Gerle <i>et al.</i>, “Structures of multisubunit membrane complexes with the CRYO ARM 200,” <i>Microscopy</i>, vol. 71, no. 5. Oxford University Press, pp. 249–261, 2022. Gerle, C., Kishikawa, J., Yamaguchi, T., Nakanishi, A., Çoruh, M. O., Makino, F., … Kato, T. (2022). Structures of multisubunit membrane complexes with the CRYO ARM 200. <i>Microscopy</i>. Oxford University Press. <a href="https://doi.org/10.1093/jmicro/dfac037">https://doi.org/10.1093/jmicro/dfac037</a> Gerle, Christoph, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” <i>Microscopy</i>, vol. 71, no. 5, Oxford University Press, 2022, pp. 249–61, doi:<a href="https://doi.org/10.1093/jmicro/dfac037">10.1093/jmicro/dfac037</a>. Gerle, Christoph, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Mehmet Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” <i>Microscopy</i>. Oxford University Press, 2022. <a href="https://doi.org/10.1093/jmicro/dfac037">https://doi.org/10.1093/jmicro/dfac037</a>. C. Gerle, J. Kishikawa, T. Yamaguchi, A. Nakanishi, M.O. Çoruh, F. Makino, T. Miyata, A. Kawamoto, K. Yokoyama, K. Namba, G. Kurisu, T. Kato, Microscopy 71 (2022) 249–261. Gerle C, Kishikawa J, Yamaguchi T, et al. Structures of multisubunit membrane complexes with the CRYO ARM 200. <i>Microscopy</i>. 2022;71(5):249-261. doi:<a href="https://doi.org/10.1093/jmicro/dfac037">10.1093/jmicro/dfac037</a> 116482022-07-25T10:04:58Z2023-08-03T12:13:37Z