TY - JOUR AB - Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica. AU - Gerle, Christoph AU - Kishikawa, Jun-ichi AU - Yamaguchi, Tomoko AU - Nakanishi, Atsuko AU - Çoruh, Mehmet Orkun AU - Makino, Fumiaki AU - Miyata, Tomoko AU - Kawamoto, Akihiro AU - Yokoyama, Ken AU - Namba, Keiichi AU - Kurisu, Genji AU - Kato, Takayuki ID - 11648 IS - 5 JF - Microscopy KW - Radiology KW - Nuclear Medicine and imaging KW - Instrumentation KW - Structural Biology SN - 2050-5698 TI - Structures of multisubunit membrane complexes with the CRYO ARM 200 VL - 71 ER -