--- _id: '11648' abstract: - lang: eng text: 'Progress in structural membrane biology has been significantly accelerated by the ongoing ''Resolution Revolution'' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica.' acknowledgement: "Cyclic Innovation for Clinical Empowerment (JP17pc0101020 from Japan Agency for Medical Research and Development (AMED) to K.N. and G.K.); Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research) from AMED (JP20am0101117 to K.N., JP16K07266 to Atsunori Oshima and C.G., JP22ama121001j0001 to Masaki Yamamoto, G.K., T.K. and C.G.); a JSPS KAHKENHI\r\ngrant (20K06514 to J.K.) and a Grant-in-aid for JSPS fellows (20J00162 to A.N.).\r\nWe are grateful for initiation and scientific support from Matthias Rogner, Marc M. Nowaczyk, Anna Frank and ̈Yuko Misumi for the PSI monomer project and also would like to thank Hideki Shigematsu for critical reading of the manuscript. And we are indebted to the two anonymous reviewers who helped us to improve our manuscript." article_processing_charge: No article_type: original author: - first_name: Christoph full_name: Gerle, Christoph last_name: Gerle - first_name: Jun-ichi full_name: Kishikawa, Jun-ichi last_name: Kishikawa - first_name: Tomoko full_name: Yamaguchi, Tomoko last_name: Yamaguchi - first_name: Atsuko full_name: Nakanishi, Atsuko last_name: Nakanishi - first_name: Mehmet Orkun full_name: Çoruh, Mehmet Orkun id: d25163e5-8d53-11eb-a251-e6dd8ea1b8ef last_name: Çoruh orcid: 0000-0002-3219-2022 - first_name: Fumiaki full_name: Makino, Fumiaki last_name: Makino - first_name: Tomoko full_name: Miyata, Tomoko last_name: Miyata - first_name: Akihiro full_name: Kawamoto, Akihiro last_name: Kawamoto - first_name: Ken full_name: Yokoyama, Ken last_name: Yokoyama - first_name: Keiichi full_name: Namba, Keiichi last_name: Namba - first_name: Genji full_name: Kurisu, Genji last_name: Kurisu - first_name: Takayuki full_name: Kato, Takayuki last_name: Kato citation: ama: Gerle C, Kishikawa J, Yamaguchi T, et al. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 2022;71(5):249-261. doi:10.1093/jmicro/dfac037 apa: Gerle, C., Kishikawa, J., Yamaguchi, T., Nakanishi, A., Çoruh, M. O., Makino, F., … Kato, T. (2022). Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. Oxford University Press. https://doi.org/10.1093/jmicro/dfac037 chicago: Gerle, Christoph, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Mehmet Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy. Oxford University Press, 2022. https://doi.org/10.1093/jmicro/dfac037. ieee: C. Gerle et al., “Structures of multisubunit membrane complexes with the CRYO ARM 200,” Microscopy, vol. 71, no. 5. Oxford University Press, pp. 249–261, 2022. ista: Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. 2022. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 71(5), 249–261. mla: Gerle, Christoph, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy, vol. 71, no. 5, Oxford University Press, 2022, pp. 249–61, doi:10.1093/jmicro/dfac037. short: C. Gerle, J. Kishikawa, T. Yamaguchi, A. Nakanishi, M.O. Çoruh, F. Makino, T. Miyata, A. Kawamoto, K. Yokoyama, K. Namba, G. Kurisu, T. Kato, Microscopy 71 (2022) 249–261. date_created: 2022-07-25T10:04:58Z date_published: 2022-10-01T00:00:00Z date_updated: 2023-08-03T12:13:37Z day: '01' ddc: - '570' department: - _id: LeSa doi: 10.1093/jmicro/dfac037 external_id: isi: - '000837950900001' pmid: - '35861182' file: - access_level: open_access checksum: 23b51c163636bf9313f7f0818312e67e content_type: application/pdf creator: dernst date_created: 2023-02-03T08:34:48Z date_updated: 2023-02-03T08:34:48Z file_id: '12498' file_name: 2022_Microscopy_Gerle.pdf file_size: 7812696 relation: main_file success: 1 file_date_updated: 2023-02-03T08:34:48Z has_accepted_license: '1' intvolume: ' 71' isi: 1 issue: '5' keyword: - Radiology - Nuclear Medicine and imaging - Instrumentation - Structural Biology language: - iso: eng month: '10' oa: 1 oa_version: Published Version page: 249-261 pmid: 1 publication: Microscopy publication_identifier: eissn: - 2050-5701 issn: - 2050-5698 publication_status: published publisher: Oxford University Press quality_controlled: '1' scopus_import: '1' status: public title: Structures of multisubunit membrane complexes with the CRYO ARM 200 tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8 volume: 71 year: '2022' ...