article published yes Rashed Abualia author 4827E134-F248-11E8-B48F-1D18A9856A870000-0002-9357-9415 Krisztina Ötvös author 29B901B0-F248-11E8-B48F-1D18A9856A870000-0002-5503-4983 Ondřej Novák author Eleonore Bouguyon author Kevin Domanegg author a24c7829-16e8-11ed-8527-c4d36ffb75390000-0002-1215-4264 Anne Krapp author Philip Nacry author Alain Gojon author Benoit Lacombe author Eva Benková author 38F4F166-F248-11E8-B48F-1D18A9856A870000-0002-8510-9739 EvBe department Hormone cross-talk drives nutrient dependent plant development project Mineral nutrition is one of the key environmental factors determining plant development and growth. Nitrate is the major form of macronutrient nitrogen that plants take up from the soil. Fluctuating availability or deficiency of this element severely limits plant growth and negatively affects crop production in the agricultural system. To cope with the heterogeneity of nitrate distribution in soil, plants evolved a complex regulatory mechanism that allows rapid adjustment of physiological and developmental processes to the status of this nutrient. The root, as a major exploitation organ that controls the uptake of nitrate to the plant body, acts as a regulatory hub that, according to nitrate availability, coordinates the growth and development of other plant organs. Here, we identified a regulatory framework, where cytokinin response factors (CRFs) play a central role as a molecular readout of the nitrate status in roots to guide shoot adaptive developmental response. We show that nitrate-driven activation of NLP7, a master regulator of nitrate response in plants, fine tunes biosynthesis of cytokinin in roots and its translocation to shoots where it enhances expression of CRFs. CRFs, through direct transcriptional regulation of PIN auxin transporters, promote the flow of auxin and thereby stimulate the development of shoot organs. https://research-explorer.ista.ac.at/download/11734/11744/2022_PNAS_Abualia.pdf application/pdfno https://creativecommons.org/licenses/by-nc-nd/4.0/ National Academy of Sciences2022 eng 1091-6490 35878040 00088149690000710.1073/pnas.2122460119 11931 R. Abualia, K. Ötvös, O. Novák, E. Bouguyon, K. Domanegg, A. Krapp, P. Nacry, A. Gojon, B. Lacombe, E. Benková, Proceedings of the National Academy of Sciences of the United States of America 119 (2022). Abualia, Rashed, et al. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31, e2122460119, National Academy of Sciences, 2022, doi:<a href="https://doi.org/10.1073/pnas.2122460119">10.1073/pnas.2122460119</a>. Abualia, Rashed, Krisztina Ötvös, Ondřej Novák, Eleonore Bouguyon, Kevin Domanegg, Anne Krapp, Philip Nacry, Alain Gojon, Benoit Lacombe, and Eva Benková. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2022. <a href="https://doi.org/10.1073/pnas.2122460119">https://doi.org/10.1073/pnas.2122460119</a>. Abualia, R., Ötvös, K., Novák, O., Bouguyon, E., Domanegg, K., Krapp, A., … Benková, E. (2022). Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.2122460119">https://doi.org/10.1073/pnas.2122460119</a> Abualia R, Ötvös K, Novák O, Bouguyon E, Domanegg K, Krapp A, Nacry P, Gojon A, Lacombe B, Benková E. 2022. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. Proceedings of the National Academy of Sciences of the United States of America. 119(31), e2122460119. R. Abualia <i>et al.</i>, “Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31. National Academy of Sciences, 2022. Abualia R, Ötvös K, Novák O, et al. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2022;119(31). doi:<a href="https://doi.org/10.1073/pnas.2122460119">10.1073/pnas.2122460119</a> 117342022-08-07T22:01:57Z2025-05-14T11:00:29Z