---
_id: '11734'
abstract:
- lang: eng
  text: Mineral nutrition is one of the key environmental factors determining plant
    development and growth. Nitrate is the major form of macronutrient nitrogen that
    plants take up from the soil. Fluctuating availability or deficiency of this element
    severely limits plant growth and negatively affects crop production in the agricultural
    system. To cope with the heterogeneity of nitrate distribution in soil, plants
    evolved a complex regulatory mechanism that allows rapid adjustment of physiological
    and developmental processes to the status of this nutrient. The root, as a major
    exploitation organ that controls the uptake of nitrate to the plant body, acts
    as a regulatory hub that, according to nitrate availability, coordinates the growth
    and development of other plant organs. Here, we identified a regulatory framework,
    where cytokinin response factors (CRFs) play a central role as a molecular readout
    of the nitrate status in roots to guide shoot adaptive developmental response.
    We show that nitrate-driven activation of NLP7, a master regulator of nitrate
    response in plants, fine tunes biosynthesis of cytokinin in roots and its translocation
    to shoots where it enhances expression of CRFs. CRFs, through direct transcriptional
    regulation of PIN auxin transporters, promote the flow of auxin and thereby stimulate
    the development of shoot organs.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We acknowledge Hana Semeradova, Juan Carlos Montesinos, Nicola Cavallari,
  Marc¸al Gallem\x03ı, Kaori Tabata, Andrej Hurn\x03y, and Sascha Waidmann for sharing
  materials; and Marina Borges Osorio for critical reading of the manuscript. Work
  in the E. Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S)
  to K.O., R.A., and E. Benkova. We acknowledge the Bioimaging Facility and Life Science
  Facilities of the Institute of Science\r\nand Technology Austria. We give sincere
  thanks to Hana Martınkova and Petra Amakorova for their help with cytokinin analyses.
  This work was funded by the Czech Science Foundation (Project No. 19-00973S)."
article_number: e2122460119
article_processing_charge: No
article_type: original
author:
- first_name: Rashed
  full_name: Abualia, Rashed
  id: 4827E134-F248-11E8-B48F-1D18A9856A87
  last_name: Abualia
  orcid: 0000-0002-9357-9415
- first_name: Krisztina
  full_name: Ötvös, Krisztina
  id: 29B901B0-F248-11E8-B48F-1D18A9856A87
  last_name: Ötvös
  orcid: 0000-0002-5503-4983
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Eleonore
  full_name: Bouguyon, Eleonore
  last_name: Bouguyon
- first_name: Kevin
  full_name: Domanegg, Kevin
  id: a24c7829-16e8-11ed-8527-c4d36ffb7539
  last_name: Domanegg
  orcid: 0000-0002-1215-4264
- first_name: Anne
  full_name: Krapp, Anne
  last_name: Krapp
- first_name: Philip
  full_name: Nacry, Philip
  last_name: Nacry
- first_name: Alain
  full_name: Gojon, Alain
  last_name: Gojon
- first_name: Benoit
  full_name: Lacombe, Benoit
  last_name: Lacombe
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
citation:
  ama: Abualia R, Ötvös K, Novák O, et al. Molecular framework integrating nitrate
    sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the
    National Academy of Sciences of the United States of America</i>. 2022;119(31).
    doi:<a href="https://doi.org/10.1073/pnas.2122460119">10.1073/pnas.2122460119</a>
  apa: Abualia, R., Ötvös, K., Novák, O., Bouguyon, E., Domanegg, K., Krapp, A., …
    Benková, E. (2022). Molecular framework integrating nitrate sensing in root and
    auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy
    of Sciences of the United States of America</i>. National Academy of Sciences.
    <a href="https://doi.org/10.1073/pnas.2122460119">https://doi.org/10.1073/pnas.2122460119</a>
  chicago: Abualia, Rashed, Krisztina Ötvös, Ondřej Novák, Eleonore Bouguyon, Kevin
    Domanegg, Anne Krapp, Philip Nacry, Alain Gojon, Benoit Lacombe, and Eva Benková.
    “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot
    Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the
    United States of America</i>. National Academy of Sciences, 2022. <a href="https://doi.org/10.1073/pnas.2122460119">https://doi.org/10.1073/pnas.2122460119</a>.
  ieee: R. Abualia <i>et al.</i>, “Molecular framework integrating nitrate sensing
    in root and auxin-guided shoot adaptive responses,” <i>Proceedings of the National
    Academy of Sciences of the United States of America</i>, vol. 119, no. 31. National
    Academy of Sciences, 2022.
  ista: Abualia R, Ötvös K, Novák O, Bouguyon E, Domanegg K, Krapp A, Nacry P, Gojon
    A, Lacombe B, Benková E. 2022. Molecular framework integrating nitrate sensing
    in root and auxin-guided shoot adaptive responses. Proceedings of the National
    Academy of Sciences of the United States of America. 119(31), e2122460119.
  mla: Abualia, Rashed, et al. “Molecular Framework Integrating Nitrate Sensing in
    Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National
    Academy of Sciences of the United States of America</i>, vol. 119, no. 31, e2122460119,
    National Academy of Sciences, 2022, doi:<a href="https://doi.org/10.1073/pnas.2122460119">10.1073/pnas.2122460119</a>.
  short: R. Abualia, K. Ötvös, O. Novák, E. Bouguyon, K. Domanegg, A. Krapp, P. Nacry,
    A. Gojon, B. Lacombe, E. Benková, Proceedings of the National Academy of Sciences
    of the United States of America 119 (2022).
corr_author: '1'
date_created: 2022-08-07T22:01:57Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2025-05-14T11:00:29Z
day: '25'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.1073/pnas.2122460119
external_id:
  isi:
  - '000881496900007'
  pmid:
  - '35878040'
file:
- access_level: open_access
  checksum: 6e97dedc281247fc3fe238a209f14af0
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  creator: dernst
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file_date_updated: 2022-08-08T07:09:58Z
has_accepted_license: '1'
intvolume: '       119'
isi: 1
issue: '31'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 1774-B16
  name: Hormone cross-talk drives nutrient dependent plant development
publication: Proceedings of the National Academy of Sciences of the United States
  of America
publication_identifier:
  eissn:
  - 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular framework integrating nitrate sensing in root and auxin-guided shoot
  adaptive responses
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 119
year: '2022'
...