article published yes N Konstantinova author Lukas Hörmayer author 2EEE7A2A-F248-11E8-B48F-1D18A9856A870000-0001-8295-2926 Matous Glanc author 1AE1EA24-02D0-11E9-9BAA-DAF4881429F20000-0003-0619-7783 R Keshkeih author Shutang Tan author 2DE75584-F248-11E8-B48F-1D18A9856A870000-0002-0471-8285 M Di Donato author K Retzer author J Moulinier-Anzola author M Schwihla author B Korbei author M Geisler author Jiří Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 C Luschnig author JiFr department Molecular mechanisms of endocytic cargo recognition in plants project Directionality in the intercellular transport of the plant hormone auxin is determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport proteins. However, apart from PIN phosphorylation at conserved motifs, no further determinants explicitly controlling polar PIN sorting decisions have been identified. Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal polarity switch in PIN2 localization in root meristem cells. WAV3 E3 ligases function as essential determinants for PIN polarity, acting independently from PINOID/WAG-dependent PIN phosphorylation. They antagonize ectopic deposition of de novo synthesized PIN proteins already immediately following completion of cell division, presumably via preventing PIN sorting into basal, ARF GEF-mediated trafficking. Our findings reveal an involvement of E3 ligases in the selective targeting of apically localized PINs in higher plants. https://research-explorer.ista.ac.at/download/12052/12063/2022_NatureCommunications_Konstantinova.pdf application/pdfno Springer Nature2022 eng 2041-1723 36050482 00084874490000410.1038/s41467-022-32888-8 13 https://doi.org/10.1038/s41467-022-33198-9 Konstantinova N, Hörmayer L, Glanc M, Keshkeih R, Tan S, Di Donato M, Retzer K, Moulinier-Anzola J, Schwihla M, Korbei B, Geisler M, Friml J, Luschnig C. 2022. WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions. Nature Communications. 13, 5147. Konstantinova, N., et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect Apical PIN Sorting Decisions.” <i>Nature Communications</i>, vol. 13, 5147, Springer Nature, 2022, doi:<a href="https://doi.org/10.1038/s41467-022-32888-8">10.1038/s41467-022-32888-8</a>. Konstantinova N, Hörmayer L, Glanc M, et al. WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions. <i>Nature Communications</i>. 2022;13. doi:<a href="https://doi.org/10.1038/s41467-022-32888-8">10.1038/s41467-022-32888-8</a> N. Konstantinova <i>et al.</i>, “WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022. Konstantinova, N., Hörmayer, L., Glanc, M., Keshkeih, R., Tan, S., Di Donato, M., … Luschnig, C. (2022). WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions. <i>Nature Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-022-32888-8">https://doi.org/10.1038/s41467-022-32888-8</a> N. Konstantinova, L. Hörmayer, M. Glanc, R. Keshkeih, S. Tan, M. Di Donato, K. Retzer, J. Moulinier-Anzola, M. Schwihla, B. Korbei, M. Geisler, J. Friml, C. Luschnig, Nature Communications 13 (2022). Konstantinova, N, Lukas Hörmayer, Matous Glanc, R Keshkeih, Shutang Tan, M Di Donato, K Retzer, et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect Apical PIN Sorting Decisions.” <i>Nature Communications</i>. Springer Nature, 2022. <a href="https://doi.org/10.1038/s41467-022-32888-8">https://doi.org/10.1038/s41467-022-32888-8</a>. 120522022-09-07T14:19:26Z2025-04-15T07:32:09Z