---
_id: '12052'
abstract:
- lang: eng
  text: Directionality in the intercellular transport of the plant hormone auxin is
    determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport
    proteins. However, apart from PIN phosphorylation at conserved motifs, no further
    determinants explicitly controlling polar PIN sorting decisions have been identified.
    Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger
    E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal
    polarity switch in PIN2 localization in root meristem cells. WAV3 E3 ligases function
    as essential determinants for PIN polarity, acting independently from PINOID/WAG-dependent
    PIN phosphorylation. They antagonize ectopic deposition of de novo synthesized
    PIN proteins already immediately following completion of cell division, presumably
    via preventing PIN sorting into basal, ARF GEF-mediated trafficking. Our findings
    reveal an involvement of E3 ligases in the selective targeting of apically localized
    PINs in higher plants.
acknowledgement: We would like to thank Tatsuo Sakai, Marcus Heisler, Toru Fujiwara,
  Lucia Strader, Christian Hardtke, Malcolm Bennett, Claus Schwechheimer, Gerd Jürgens
  and Remko Offringa for sharing published materials and Alba Grau Gimeno for support.
  We are greatly indebted to Bert de Rybel for supporting N.K. and M.G. to work on
  the final stages of manuscript preparation as postdocs in his laboratory. A full-length
  SOR1 cDNA clone (J090099M14) was obtained from the National Agriculture and Food
  Research Organization (NARO, Japan). Support by the Multiscale Imaging Core Facility
  at the BOKU is greatly acknowledged. This work has been supported by grants from
  the Austrian Science Fund (FWF P25931-B16; P31493-B25 to Christian Luschnig; I3630-B25
  to Jiří Friml; P30850-B32 to Barbara Korbei) and from the Swiss National Funds (31003A-165877/1
  to Markus Geisler) and the European Union’s Horizon 2020 research and innovation
  program (Marie Skłodowska-Curie grant agreement No 885979 to Matouš Glanc).
article_number: '5147'
article_processing_charge: No
article_type: original
author:
- first_name: N
  full_name: Konstantinova, N
  last_name: Konstantinova
- first_name: Lukas
  full_name: Hörmayer, Lukas
  id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
  last_name: Hörmayer
  orcid: 0000-0001-8295-2926
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: R
  full_name: Keshkeih, R
  last_name: Keshkeih
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: M
  full_name: Di Donato, M
  last_name: Di Donato
- first_name: K
  full_name: Retzer, K
  last_name: Retzer
- first_name: J
  full_name: Moulinier-Anzola, J
  last_name: Moulinier-Anzola
- first_name: M
  full_name: Schwihla, M
  last_name: Schwihla
- first_name: B
  full_name: Korbei, B
  last_name: Korbei
- first_name: M
  full_name: Geisler, M
  last_name: Geisler
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: C
  full_name: Luschnig, C
  last_name: Luschnig
citation:
  ama: Konstantinova N, Hörmayer L, Glanc M, et al. WAVY GROWTH Arabidopsis E3 ubiquitin
    ligases affect apical PIN sorting decisions. <i>Nature Communications</i>. 2022;13.
    doi:<a href="https://doi.org/10.1038/s41467-022-32888-8">10.1038/s41467-022-32888-8</a>
  apa: Konstantinova, N., Hörmayer, L., Glanc, M., Keshkeih, R., Tan, S., Di Donato,
    M., … Luschnig, C. (2022). WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect
    apical PIN sorting decisions. <i>Nature Communications</i>. Springer Nature. <a
    href="https://doi.org/10.1038/s41467-022-32888-8">https://doi.org/10.1038/s41467-022-32888-8</a>
  chicago: Konstantinova, N, Lukas Hörmayer, Matous Glanc, R Keshkeih, Shutang Tan,
    M Di Donato, K Retzer, et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
    Apical PIN Sorting Decisions.” <i>Nature Communications</i>. Springer Nature,
    2022. <a href="https://doi.org/10.1038/s41467-022-32888-8">https://doi.org/10.1038/s41467-022-32888-8</a>.
  ieee: N. Konstantinova <i>et al.</i>, “WAVY GROWTH Arabidopsis E3 ubiquitin ligases
    affect apical PIN sorting decisions,” <i>Nature Communications</i>, vol. 13. Springer
    Nature, 2022.
  ista: Konstantinova N, Hörmayer L, Glanc M, Keshkeih R, Tan S, Di Donato M, Retzer
    K, Moulinier-Anzola J, Schwihla M, Korbei B, Geisler M, Friml J, Luschnig C. 2022.
    WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.
    Nature Communications. 13, 5147.
  mla: Konstantinova, N., et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
    Apical PIN Sorting Decisions.” <i>Nature Communications</i>, vol. 13, 5147, Springer
    Nature, 2022, doi:<a href="https://doi.org/10.1038/s41467-022-32888-8">10.1038/s41467-022-32888-8</a>.
  short: N. Konstantinova, L. Hörmayer, M. Glanc, R. Keshkeih, S. Tan, M. Di Donato,
    K. Retzer, J. Moulinier-Anzola, M. Schwihla, B. Korbei, M. Geisler, J. Friml,
    C. Luschnig, Nature Communications 13 (2022).
date_created: 2022-09-07T14:19:26Z
date_published: 2022-09-01T00:00:00Z
date_updated: 2025-04-15T07:32:09Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-022-32888-8
external_id:
  isi:
  - '000848744900004'
  pmid:
  - '36050482'
file:
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language:
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month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Nature Communications
publication_identifier:
  issn:
  - 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.1038/s41467-022-33198-9
scopus_import: '1'
status: public
title: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...