---
_id: '1212'
abstract:
- lang: eng
text: 'Plants adjust their growth according to gravity. Gravitropism involves gravity
perception, signal transduction, and asymmetric growth response, with organ bending
as a consequence [1]. Asymmetric growth results from the asymmetric distribution
of the plant-specific signaling molecule auxin [2] that is generated by lateral
transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3
(PIN3) [3–5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal
cells, correlating with increased auxin accumulation in adjacent tissues at the
lower side of the stimulated organ, where auxin induces cell elongation and, hence,
organ bending. A curvature response allows the hypocotyl to resume straight growth
at a defined angle [6], implying that at some point auxin symmetry is restored
to prevent overbending. Here, we present initial insights into cellular and molecular
mechanisms that lead to the termination of the tropic response. We identified
an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry
of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization
events redirect auxin fluxes at different time points of the gravity response:
first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower
hypocotyl side, where auxin gradually accumulates and promotes growth, and later
PIN3 polarization to the opposite cell side, depleting this auxin maximum to end
the bending. Accordingly, genetic or pharmacological interference with the late
PIN3 polarization prevents termination of the response and leads to hypocotyl
overbending. This observation reveals a role of auxin feedback on PIN polarity
in the termination of the tropic response. © 2016 Elsevier Ltd'
acknowledgement: "We thank Dr. Jie Li (Key Laboratory of Plant Molecular Physiology,
Chinese Academy of Science, China) for the pPIN3::PIN3-GFP/DII::VENUS line and Martine
De Cock for help in preparing the manuscript. This work was supported by the European
Research Council (project ERC-2011-StG-20101109-PSDP), by the Czech Science Foundation
GAČR (GA13-40637S) to J.F., and by the Ministry of Education, Youth and Sports of
the Czech Republic under the project CEITEC 2020 (LQ1601) to H.S.R. H.R. is indebted
to the Agency for Innovation by Science and Technology (IWT) for a predoctoral fellowship.\r\n"
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Siyuan
full_name: Song, Siyuan
last_name: Song
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. Termination of shoot
gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
2016;26(22):3026-3032. doi:10.1016/j.cub.2016.08.067
apa: Rakusová, H., Abbas, M., Han, H., Song, S., Robert, H., & Friml, J. (2016).
Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity.
Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2016.08.067
chicago: Rakusová, Hana, Mohamad Abbas, Huibin Han, Siyuan Song, Hélène Robert,
and Jiří Friml. “Termination of Shoot Gravitropic Responses by Auxin Feedback
on PIN3 Polarity.” Current Biology. Cell Press, 2016. https://doi.org/10.1016/j.cub.2016.08.067.
ieee: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, and J. Friml, “Termination
of shoot gravitropic responses by auxin feedback on PIN3 polarity,” Current
Biology, vol. 26, no. 22. Cell Press, pp. 3026–3032, 2016.
ista: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. 2016. Termination of
shoot gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
26(22), 3026–3032.
mla: Rakusová, Hana, et al. “Termination of Shoot Gravitropic Responses by Auxin
Feedback on PIN3 Polarity.” Current Biology, vol. 26, no. 22, Cell Press,
2016, pp. 3026–32, doi:10.1016/j.cub.2016.08.067.
short: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology
26 (2016) 3026–3032.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-21T00:00:00Z
date_updated: 2021-01-12T06:49:08Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2016.08.067
ec_funded: 1
file:
- access_level: open_access
checksum: 79ed2498185a027cf51a8f88100379e6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:33Z
date_updated: 2020-07-14T12:44:39Z
file_id: '4757'
file_name: IST-2018-1008-v1+1_Rakusova_CurrBiol_2016_proof.pdf
file_size: 5391923
relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: ' 26'
issue: '22'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 3026 - 3032
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '6138'
pubrep_id: '1008'
quality_controlled: '1'
scopus_import: 1
status: public
title: Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...