--- res: bibo_abstract: - Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes with the multivesicular body-localized ESCRT-I component VPS23A, leading to the formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation. Altogether, our results reveal a conserved vacuolar sorting hub that regulates autophagic flux in plants.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jierui foaf_name: Zhao, Jierui foaf_surname: Zhao - foaf_Person: foaf_givenName: Mai Thu foaf_name: Bui, Mai Thu foaf_surname: Bui - foaf_Person: foaf_givenName: Juncai foaf_name: Ma, Juncai foaf_surname: Ma - foaf_Person: foaf_givenName: Fabian foaf_name: Künzl, Fabian foaf_surname: Künzl - foaf_Person: foaf_givenName: Lorenzo foaf_name: Picchianti, Lorenzo foaf_surname: Picchianti - foaf_Person: foaf_givenName: Juan Carlos foaf_name: De La Concepcion, Juan Carlos foaf_surname: De La Concepcion - foaf_Person: foaf_givenName: Yixuan foaf_name: Chen, Yixuan foaf_surname: Chen - foaf_Person: foaf_givenName: Sofia foaf_name: Petsangouraki, Sofia foaf_surname: Petsangouraki - foaf_Person: foaf_givenName: Azadeh foaf_name: Mohseni, Azadeh foaf_surname: Mohseni - foaf_Person: foaf_givenName: Marta foaf_name: García-Leon, Marta foaf_surname: García-Leon - foaf_Person: foaf_givenName: Marta Salas foaf_name: Gomez, Marta Salas foaf_surname: Gomez - foaf_Person: foaf_givenName: Caterina foaf_name: Giannini, Caterina foaf_surname: Giannini foaf_workInfoHomepage: http://www.librecat.org/personId=e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 - foaf_Person: foaf_givenName: Dubois foaf_name: Gwennogan, Dubois foaf_surname: Gwennogan - foaf_Person: foaf_givenName: Roksolana foaf_name: Kobylinska, Roksolana foaf_surname: Kobylinska - foaf_Person: foaf_givenName: Marion foaf_name: Clavel, Marion foaf_surname: Clavel - foaf_Person: foaf_givenName: Swen foaf_name: Schellmann, Swen foaf_surname: Schellmann - foaf_Person: foaf_givenName: Yvon foaf_name: Jaillais, Yvon foaf_surname: Jaillais - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Byung-Ho foaf_name: Kang, Byung-Ho foaf_surname: Kang - foaf_Person: foaf_givenName: Yasin foaf_name: Dagdas, Yasin foaf_surname: Dagdas bibo_doi: 10.1083/jcb.202203139 bibo_issue: '12' bibo_volume: 221 dct_date: 2022^xs_gYear dct_identifier: - UT:000932958800001 dct_isPartOf: - http://id.crossref.org/issn/0021-9525 - http://id.crossref.org/issn/1540-8140 dct_language: eng dct_publisher: Rockefeller University Press@ dct_subject: - Cell Biology dct_title: Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole@ fabio_hasPubmedId: '36260289' ...