---
res:
  bibo_abstract:
  - Bacterial cytokinesis is commonly initiated by the Z-ring, a dynamic cytoskeletal
    structure that assembles at the site of division. Its primary component is FtsZ,
    a tubulin-like GTPase, that like its eukaryotic relative forms protein filaments
    in the presence of GTP. Since the discovery of the Z-ring 25 years ago, various
    models for the role of FtsZ have been suggested. However, important information
    about the architecture and dynamics of FtsZ filaments during cytokinesis is still
    missing. One reason for this lack of knowledge has been the small size of bacteria,
    which has made it difficult to resolve the orientation and dynamics of individual
    FtsZ filaments in the Z-ring. While superresolution microscopy experiments have
    helped to gain more information about the organization of the Z-ring in the dividing
    cell, they were not yet able to elucidate a mechanism of how FtsZ filaments reorganize
    during assembly and disassembly of the Z-ring. In this chapter, we explain how
    to use an in vitro reconstitution approach to investigate the self-organization
    of FtsZ filaments recruited to a biomimetic lipid bilayer by its membrane anchor
    FtsA. We show how to perform single-molecule experiments to study the behavior
    of individual FtsZ monomers during the constant reorganization of the FtsZ-FtsA
    filament network. We describe how to analyze the dynamics of single molecules
    and explain why this information can help to shed light onto possible mechanism
    of Z-ring constriction. We believe that similar experimental approaches will be
    useful to study the mechanism of membrane-based polymerization of other cytoskeletal
    systems, not only from prokaryotic but also eukaryotic origin.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Natalia
      foaf_name: Baranova, Natalia
      foaf_surname: Baranova
      foaf_workInfoHomepage: http://www.librecat.org/personId=38661662-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-3086-9124
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Loose, Martin
      foaf_surname: Loose
      foaf_workInfoHomepage: http://www.librecat.org/personId=462D4284-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7309-9724
  bibo_doi: 10.1016/bs.mcb.2016.03.036
  bibo_volume: 137
  dct_date: 2017^xs_gYear
  dct_identifier:
  - UT:000403542900022
  dct_isPartOf:
  - http://id.crossref.org/issn/0091-679X
  dct_language: eng
  dct_publisher: Academic Press@
  dct_title: Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA
    copolymers@
...