---
_id: '12152'
abstract:
- lang: eng
  text: ESCRT-III filaments are composite cytoskeletal polymers that can constrict
    and cut cell membranes from the inside of the membrane neck. Membrane-bound ESCRT-III
    filaments undergo a series of dramatic composition and geometry changes in the
    presence of an ATP-consuming Vps4 enzyme, which causes stepwise changes in the
    membrane morphology. We set out to understand the physical mechanisms involved
    in translating the changes in ESCRT-III polymer composition into membrane deformation.
    We have built a coarse-grained model in which ESCRT-III polymers of different
    geometries and mechanical properties are allowed to copolymerise and bind to a
    deformable membrane. By modelling ATP-driven stepwise depolymerisation of specific
    polymers, we identify mechanical regimes in which changes in filament composition
    trigger the associated membrane transition from a flat to a buckled state, and
    then to a tubule state that eventually undergoes scission to release a small cargo-loaded
    vesicle. We then characterise how the location and kinetics of polymer loss affects
    the extent of membrane deformation and the efficiency of membrane neck scission.
    Our results identify the near-minimal mechanical conditions for the operation
    of shape-shifting composite polymers that sever membrane necks.
acknowledgement: "A.S . received an award from European Research Council (https://erc.europa.eu,
  “NEPA\"\r\n802960), and an award from the Royal Society (https://royalsociety.org,
  UF160266). L. H.-K.\r\nreceived an award from the Biotechnology and Biological Sciences
  Research Council (https://\r\nwww.ukri.org/councils/bbsrc/). E. L. received an award
  from the University College London (https://www.ucl.ac.uk/biophysics/news/2022/feb/applications-biop-brian-duff-and-ipls-summerundergraduate-studentships-now-open,
  Brian Duff Undergraduate Summer Research Studentship). B.B. and A.S. received an
  award from Volkswagen Foundation https://www.volkswagenstiftung.de/en/foundation,
  Az 96727), and an award from Medical Research Council (https://www.ukri.org/councils/mrc,
  MC_CF1226). A. R. received an\r\naward from the Swiss National Fund for Research
  (https://www.snf.ch/en, 31003A_130520,\r\n31003A_149975, and 31003A_173087) and
  an award from the European Research Council\r\nConsolidator (https://erc.europa.eu,
  311536). The funders had no role in study design, data collection and analysis,
  decision to publish, or preparation of the manuscript."
article_number: e1010586
article_processing_charge: No
article_type: original
author:
- first_name: Xiuyun
  full_name: Jiang, Xiuyun
  last_name: Jiang
- first_name: Lena
  full_name: Harker-Kirschneck, Lena
  last_name: Harker-Kirschneck
- first_name: Christian Eduardo
  full_name: Vanhille-Campos, Christian Eduardo
  id: 3adeca52-9313-11ed-b1ac-c170b2505714
  last_name: Vanhille-Campos
- first_name: Anna-Katharina
  full_name: Pfitzner, Anna-Katharina
  last_name: Pfitzner
- first_name: Elene
  full_name: Lominadze, Elene
  last_name: Lominadze
- first_name: Aurélien
  full_name: Roux, Aurélien
  last_name: Roux
- first_name: Buzz
  full_name: Baum, Buzz
  last_name: Baum
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
citation:
  ama: Jiang X, Harker-Kirschneck L, Vanhille-Campos CE, et al. Modelling membrane
    reshaping by staged polymerization of ESCRT-III filaments. <i>PLOS Computational
    Biology</i>. 2022;18(10). doi:<a href="https://doi.org/10.1371/journal.pcbi.1010586">10.1371/journal.pcbi.1010586</a>
  apa: Jiang, X., Harker-Kirschneck, L., Vanhille-Campos, C. E., Pfitzner, A.-K.,
    Lominadze, E., Roux, A., … Šarić, A. (2022). Modelling membrane reshaping by staged
    polymerization of ESCRT-III filaments. <i>PLOS Computational Biology</i>. Public
    Library of Science. <a href="https://doi.org/10.1371/journal.pcbi.1010586">https://doi.org/10.1371/journal.pcbi.1010586</a>
  chicago: Jiang, Xiuyun, Lena Harker-Kirschneck, Christian Eduardo Vanhille-Campos,
    Anna-Katharina Pfitzner, Elene Lominadze, Aurélien Roux, Buzz Baum, and Anđela
    Šarić. “Modelling Membrane Reshaping by Staged Polymerization of ESCRT-III Filaments.”
    <i>PLOS Computational Biology</i>. Public Library of Science, 2022. <a href="https://doi.org/10.1371/journal.pcbi.1010586">https://doi.org/10.1371/journal.pcbi.1010586</a>.
  ieee: X. Jiang <i>et al.</i>, “Modelling membrane reshaping by staged polymerization
    of ESCRT-III filaments,” <i>PLOS Computational Biology</i>, vol. 18, no. 10. Public
    Library of Science, 2022.
  ista: Jiang X, Harker-Kirschneck L, Vanhille-Campos CE, Pfitzner A-K, Lominadze
    E, Roux A, Baum B, Šarić A. 2022. Modelling membrane reshaping by staged polymerization
    of ESCRT-III filaments. PLOS Computational Biology. 18(10), e1010586.
  mla: Jiang, Xiuyun, et al. “Modelling Membrane Reshaping by Staged Polymerization
    of ESCRT-III Filaments.” <i>PLOS Computational Biology</i>, vol. 18, no. 10, e1010586,
    Public Library of Science, 2022, doi:<a href="https://doi.org/10.1371/journal.pcbi.1010586">10.1371/journal.pcbi.1010586</a>.
  short: X. Jiang, L. Harker-Kirschneck, C.E. Vanhille-Campos, A.-K. Pfitzner, E.
    Lominadze, A. Roux, B. Baum, A. Šarić, PLOS Computational Biology 18 (2022).
corr_author: '1'
date_created: 2023-01-12T12:08:10Z
date_published: 2022-10-17T00:00:00Z
date_updated: 2025-06-12T06:19:28Z
day: '17'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1371/journal.pcbi.1010586
ec_funded: 1
external_id:
  isi:
  - '000924885500005'
  pmid:
  - '36251703'
file:
- access_level: open_access
  checksum: bada6a7865e470cf42bbdfa67dd471d2
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-24T10:45:01Z
  date_updated: 2023-01-24T10:45:01Z
  file_id: '12359'
  file_name: 2022_PLoSCompBio_Jiang.pdf
  file_size: 2641067
  relation: main_file
  success: 1
file_date_updated: 2023-01-24T10:45:01Z
fulldoi: https://doi.org/10.1371/journal.pcbi.1010586
has_accepted_license: '1'
intvolume: '        18'
isi: 1
issue: '10'
keyword:
- Computational Theory and Mathematics
- Cellular and Molecular Neuroscience
- Genetics
- Molecular Biology
- Ecology
- Modeling and Simulation
- Ecology
- Evolution
- Behavior and Systematics
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
  call_identifier: H2020
  grant_number: '802960'
  name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: eba0f67c-77a9-11ec-83b8-cc8501b3e222
  grant_number: '96752'
  name: 'The evolution of trafficking: from archaea to eukaryotes'
publication: PLOS Computational Biology
publication_identifier:
  issn:
  - 1553-7358
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: https://github.com/sharonJXY/3-filament-model
scopus_import: '1'
status: public
title: Modelling membrane reshaping by staged polymerization of ESCRT-III filaments
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2022'
...