{"year":"2022","doi":"10.1080/19490976.2022.2143218","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","keyword":["Infectious Diseases","Microbiology (medical)","Gastroenterology","Microbiology"],"article_number":"e2143218","external_id":{"isi":["000889180100001"]},"date_published":"2022-11-22T00:00:00Z","date_created":"2023-01-12T12:11:36Z","abstract":[{"lang":"eng","text":"With increasing urbanization and industrialization, the prevalence of inflammatory bowel diseases (IBDs) has steadily been rising over the past two decades. IBD involves flares of gastrointestinal (GI) inflammation accompanied by microbiota perturbations. However, microbial mechanisms that trigger such flares remain elusive. Here, we analyzed the association of the emerging pathogen atypical enteropathogenic E. coli (aEPEC) with IBD disease activity. The presence of diarrheagenic E. coli was assessed in stool samples from 630 IBD patients and 234 age- and sex-matched controls without GI symptoms. Microbiota was analyzed with 16S ribosomal RNA gene amplicon sequencing, and 57 clinical aEPEC isolates were subjected to whole-genome sequencing and in vitro pathogenicity experiments including biofilm formation, epithelial barrier function and the ability to induce pro-inflammatory signaling. The presence of aEPEC correlated with laboratory, clinical and endoscopic disease activity in ulcerative colitis (UC), as well as microbiota dysbiosis. In vitro, aEPEC strains induce epithelial p21-activated kinases, disrupt the epithelial barrier and display potent biofilm formation. The effector proteins espV and espG2 distinguish aEPEC cultured from UC and Crohn’s disease patients, respectively. EspV-positive aEPEC harbor more virulence factors and have a higher pro-inflammatory potential, which is counteracted by 5-ASA. aEPEC may tip a fragile immune–microbiota homeostasis and thereby contribute to flares in UC. aEPEC isolates from UC patients display properties to disrupt the epithelial barrier and to induce pro-inflammatory signaling in vitro."}],"quality_controlled":"1","acknowledgement":"We would like to acknowledge Anita Krnjic, Christina Gmainer, Marion Nehr, Helga Mock, and Sena Ecin for technical support in conducting the experiments.\r\nThis study was supported by the Austrian Science Fund (P 32302) and the Vienna Science and Technology Fund (LS18- 053; Austrian Science Fund (FWF)) [P 32302].","title":"Atypical enteropathogenic E. coli are associated with disease activity in ulcerative colitis","language":[{"iso":"eng"}],"day":"22","issue":"1","month":"11","oa_version":"Published Version","publication":"Gut Microbes","publisher":"Taylor & Francis","license":"https://creativecommons.org/licenses/by/4.0/","has_accepted_license":"1","file_date_updated":"2023-01-26T10:56:51Z","article_type":"original","file":[{"date_updated":"2023-01-26T10:56:51Z","file_name":"2022_GutMicrobes_Baumgartner.pdf","access_level":"open_access","file_id":"12400","checksum":"ee7681a17ae27645e9b5c1df61c15429","creator":"dernst","content_type":"application/pdf","file_size":4075251,"success":1,"relation":"main_file","date_created":"2023-01-26T10:56:51Z"}],"publication_status":"published","article_processing_charge":"No","author":[{"full_name":"Baumgartner, Maximilian","last_name":"Baumgartner","first_name":"Maximilian"},{"full_name":"Zirnbauer, Rebecca","first_name":"Rebecca","last_name":"Zirnbauer"},{"full_name":"Schlager, Sabine","last_name":"Schlager","first_name":"Sabine"},{"first_name":"Daniel","last_name":"Mertens","full_name":"Mertens, Daniel"},{"first_name":"Nikolaus","last_name":"Gasche","full_name":"Gasche, Nikolaus"},{"first_name":"Barbara","last_name":"Sladek","full_name":"Sladek, Barbara"},{"full_name":"Herbold, Craig","last_name":"Herbold","first_name":"Craig"},{"last_name":"Bochkareva","first_name":"Olga","full_name":"Bochkareva, Olga"},{"full_name":"Emelianenko, Vera","id":"20152b9d-927a-11ed-8107-be36d740812d","last_name":"Emelianenko","first_name":"Vera"},{"full_name":"Vogelsang, Harald","last_name":"Vogelsang","first_name":"Harald"},{"first_name":"Michaela","last_name":"Lang","full_name":"Lang, Michaela"},{"full_name":"Klotz, Anton","first_name":"Anton","last_name":"Klotz"},{"last_name":"Moik","first_name":"Birgit","full_name":"Moik, Birgit"},{"last_name":"Makristathis","first_name":"Athanasios","full_name":"Makristathis, Athanasios"},{"last_name":"Berry","first_name":"David","full_name":"Berry, David"},{"full_name":"Dabsch, Stefanie","first_name":"Stefanie","last_name":"Dabsch"},{"full_name":"Khare, Vineeta","last_name":"Khare","first_name":"Vineeta"},{"full_name":"Gasche, Christoph","last_name":"Gasche","first_name":"Christoph"}],"ddc":["570"],"isi":1,"status":"public","_id":"12173","citation":{"short":"M. Baumgartner, R. Zirnbauer, S. Schlager, D. Mertens, N. Gasche, B. Sladek, C. Herbold, O. Bochkareva, V. Emelianenko, H. Vogelsang, M. Lang, A. Klotz, B. Moik, A. Makristathis, D. Berry, S. Dabsch, V. Khare, C. Gasche, Gut Microbes 14 (2022).","mla":"Baumgartner, Maximilian, et al. “Atypical Enteropathogenic E. Coli Are Associated with Disease Activity in Ulcerative Colitis.” <i>Gut Microbes</i>, vol. 14, no. 1, e2143218, Taylor &#38; Francis, 2022, doi:<a href=\"https://doi.org/10.1080/19490976.2022.2143218\">10.1080/19490976.2022.2143218</a>.","apa":"Baumgartner, M., Zirnbauer, R., Schlager, S., Mertens, D., Gasche, N., Sladek, B., … Gasche, C. (2022). Atypical enteropathogenic E. coli are associated with disease activity in ulcerative colitis. <i>Gut Microbes</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.1080/19490976.2022.2143218\">https://doi.org/10.1080/19490976.2022.2143218</a>","ieee":"M. Baumgartner <i>et al.</i>, “Atypical enteropathogenic E. coli are associated with disease activity in ulcerative colitis,” <i>Gut Microbes</i>, vol. 14, no. 1. Taylor &#38; Francis, 2022.","chicago":"Baumgartner, Maximilian, Rebecca Zirnbauer, Sabine Schlager, Daniel Mertens, Nikolaus Gasche, Barbara Sladek, Craig Herbold, et al. “Atypical Enteropathogenic E. Coli Are Associated with Disease Activity in Ulcerative Colitis.” <i>Gut Microbes</i>. Taylor &#38; Francis, 2022. <a href=\"https://doi.org/10.1080/19490976.2022.2143218\">https://doi.org/10.1080/19490976.2022.2143218</a>.","ama":"Baumgartner M, Zirnbauer R, Schlager S, et al. Atypical enteropathogenic E. coli are associated with disease activity in ulcerative colitis. <i>Gut Microbes</i>. 2022;14(1). doi:<a href=\"https://doi.org/10.1080/19490976.2022.2143218\">10.1080/19490976.2022.2143218</a>","ista":"Baumgartner M, Zirnbauer R, Schlager S, Mertens D, Gasche N, Sladek B, Herbold C, Bochkareva O, Emelianenko V, Vogelsang H, Lang M, Klotz A, Moik B, Makristathis A, Berry D, Dabsch S, Khare V, Gasche C. 2022. Atypical enteropathogenic E. coli are associated with disease activity in ulcerative colitis. Gut Microbes. 14(1), e2143218."},"publication_identifier":{"eissn":["1949-0984"],"issn":["1949-0976"]},"oa":1,"type":"journal_article","scopus_import":"1","date_updated":"2023-08-04T09:10:18Z","department":[{"_id":"FyKo"}],"intvolume":"        14","volume":14}