--- res: bibo_abstract: - Investigating the physiology of cyanobacteria cultured under a diel light regime is relevant for a better understanding of the resulting growth characteristics and for specific biotechnological applications that are foreseen for these photosynthetic organisms. Here, we present the results of a multiomics study of the model cyanobacterium Synechocystis sp. strain PCC 6803, cultured in a lab-scale photobioreactor in physiological conditions relevant for large-scale culturing. The culture was sparged withN2 andCO2, leading to an anoxic environment during the dark period. Growth followed the availability of light. Metabolite analysis performed with 1Hnuclear magnetic resonance analysis showed that amino acids involved in nitrogen and sulfur assimilation showed elevated levels in the light. Most protein levels, analyzed through mass spectrometry, remained rather stable. However, several high-light-response proteins and stress-response proteins showed distinct changes at the onset of the light period. Microarray-based transcript analysis found common patterns of~56% of the transcriptome following the diel regime. These oscillating transcripts could be grouped coarsely into genes that were upregulated and downregulated in the dark period. The accumulated glycogen was degraded in the anaerobic environment in the dark. A small part was degraded gradually, reflecting basic maintenance requirements of the cells in darkness. Surprisingly, the largest part was degraded rapidly in a short time span at the end of the dark period. This degradation could allow rapid formation of metabolic intermediates at the end of the dark period, preparing the cells for the resumption of growth at the start of the light period.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Andreas foaf_name: Angermayr, Andreas foaf_surname: Angermayr foaf_workInfoHomepage: http://www.librecat.org/personId=4677C796-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8619-2223 - foaf_Person: foaf_givenName: Pascal foaf_name: Van Alphen, Pascal foaf_surname: Van Alphen - foaf_Person: foaf_givenName: Dicle foaf_name: Hasdemir, Dicle foaf_surname: Hasdemir - foaf_Person: foaf_givenName: Gertjan foaf_name: Kramer, Gertjan foaf_surname: Kramer - foaf_Person: foaf_givenName: Muzamal foaf_name: Iqbal, Muzamal foaf_surname: Iqbal - foaf_Person: foaf_givenName: Wilmar foaf_name: Van Grondelle, Wilmar foaf_surname: Van Grondelle - foaf_Person: foaf_givenName: Huub foaf_name: Hoefsloot, Huub foaf_surname: Hoefsloot - foaf_Person: foaf_givenName: Younghae foaf_name: Choi, Younghae foaf_surname: Choi - foaf_Person: foaf_givenName: Klaas foaf_name: Hellingwerf, Klaas foaf_surname: Hellingwerf bibo_doi: 10.1128/AEM.00256-16 bibo_issue: '14' bibo_volume: 82 dct_date: 2016^xs_gYear dct_language: eng dct_publisher: American Society for Microbiology@ dct_title: Culturing synechocystis sp. Strain pcc 6803 with N2 and CO2 in a diel regime reveals multiphase glycogen dynamics with low maintenance costs@ ...