---
_id: '12189'
abstract:
- lang: eng
  text: Meiotic crossovers (COs) are important for reshuffling genetic information
    between homologous chromosomes and they are essential for their correct segregation.
    COs are unevenly distributed along chromosomes and the underlying mechanisms controlling
    CO localization are not well understood. We previously showed that meiotic COs
    are mis-localized in the absence of AXR1, an enzyme involved in the neddylation/rubylation
    protein modification pathway in Arabidopsis thaliana. Here, we report that in
    axr1-/-, male meiocytes show a strong defect in chromosome pairing whereas the
    formation of the telomere bouquet is not affected. COs are also redistributed
    towards subtelomeric chromosomal ends where they frequently form clusters, in
    contrast to large central regions depleted in recombination. The CO suppressed
    regions correlate with DNA hypermethylation of transposable elements (TEs) in
    the CHH context in axr1-/- meiocytes. Through examining somatic methylomes, we
    found axr1-/- affects DNA methylation in a plant, causing hypermethylation in
    all sequence contexts (CG, CHG and CHH) in TEs. Impairment of the main pathways
    involved in DNA methylation is epistatic over axr1-/- for DNA methylation in somatic
    cells but does not restore regular chromosome segregation during meiosis. Collectively,
    our findings reveal that the neddylation pathway not only regulates hormonal perception
    and CO distribution but is also, directly or indirectly, a major limiting pathway
    of TE DNA methylation in somatic cells.
acknowledgement: The authors wish to thank Cécile Raynaud, Eric Jenczewski, Rajeev
  Kumar, Raphaël Mercier and Jean Molinier for critical reading of the manuscript.
article_number: e1008894
article_processing_charge: No
article_type: original
author:
- first_name: Nicolas
  full_name: Christophorou, Nicolas
  last_name: Christophorou
- first_name: Wenjing
  full_name: She, Wenjing
  last_name: She
- first_name: Jincheng
  full_name: Long, Jincheng
  last_name: Long
- first_name: Aurélie
  full_name: Hurel, Aurélie
  last_name: Hurel
- first_name: Sébastien
  full_name: Beaubiat, Sébastien
  last_name: Beaubiat
- first_name: Yassir
  full_name: Idir, Yassir
  last_name: Idir
- first_name: Marina
  full_name: Tagliaro-Jahns, Marina
  last_name: Tagliaro-Jahns
- first_name: Aurélie
  full_name: Chambon, Aurélie
  last_name: Chambon
- first_name: Victor
  full_name: Solier, Victor
  last_name: Solier
- first_name: Daniel
  full_name: Vezon, Daniel
  last_name: Vezon
- first_name: Mathilde
  full_name: Grelon, Mathilde
  last_name: Grelon
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Nicolas
  full_name: Bouché, Nicolas
  last_name: Bouché
- first_name: Christine
  full_name: Mézard, Christine
  last_name: Mézard
citation:
  ama: Christophorou N, She W, Long J, et al. AXR1 affects DNA methylation independently
    of its role in regulating meiotic crossover localization. <i>PLOS Genetics</i>.
    2020;16(6). doi:<a href="https://doi.org/10.1371/journal.pgen.1008894">10.1371/journal.pgen.1008894</a>
  apa: Christophorou, N., She, W., Long, J., Hurel, A., Beaubiat, S., Idir, Y., …
    Mézard, C. (2020). AXR1 affects DNA methylation independently of its role in regulating
    meiotic crossover localization. <i>PLOS Genetics</i>. Public Library of Science
    (PLoS). <a href="https://doi.org/10.1371/journal.pgen.1008894">https://doi.org/10.1371/journal.pgen.1008894</a>
  chicago: Christophorou, Nicolas, Wenjing She, Jincheng Long, Aurélie Hurel, Sébastien
    Beaubiat, Yassir Idir, Marina Tagliaro-Jahns, et al. “AXR1 Affects DNA Methylation
    Independently of Its Role in Regulating Meiotic Crossover Localization.” <i>PLOS
    Genetics</i>. Public Library of Science (PLoS), 2020. <a href="https://doi.org/10.1371/journal.pgen.1008894">https://doi.org/10.1371/journal.pgen.1008894</a>.
  ieee: N. Christophorou <i>et al.</i>, “AXR1 affects DNA methylation independently
    of its role in regulating meiotic crossover localization,” <i>PLOS Genetics</i>,
    vol. 16, no. 6. Public Library of Science (PLoS), 2020.
  ista: Christophorou N, She W, Long J, Hurel A, Beaubiat S, Idir Y, Tagliaro-Jahns
    M, Chambon A, Solier V, Vezon D, Grelon M, Feng X, Bouché N, Mézard C. 2020. AXR1
    affects DNA methylation independently of its role in regulating meiotic crossover
    localization. PLOS Genetics. 16(6), e1008894.
  mla: Christophorou, Nicolas, et al. “AXR1 Affects DNA Methylation Independently
    of Its Role in Regulating Meiotic Crossover Localization.” <i>PLOS Genetics</i>,
    vol. 16, no. 6, e1008894, Public Library of Science (PLoS), 2020, doi:<a href="https://doi.org/10.1371/journal.pgen.1008894">10.1371/journal.pgen.1008894</a>.
  short: N. Christophorou, W. She, J. Long, A. Hurel, S. Beaubiat, Y. Idir, M. Tagliaro-Jahns,
    A. Chambon, V. Solier, D. Vezon, M. Grelon, X. Feng, N. Bouché, C. Mézard, PLOS
    Genetics 16 (2020).
date_created: 2023-01-16T09:16:10Z
date_published: 2020-06-29T00:00:00Z
date_updated: 2023-05-08T10:54:39Z
day: '29'
department:
- _id: XiFe
doi: 10.1371/journal.pgen.1008894
extern: '1'
external_id:
  pmid:
  - '32598340'
intvolume: '        16'
issue: '6'
keyword:
- Cancer Research
- Genetics (clinical)
- Genetics
- Molecular Biology
- Ecology
- Evolution
- Behavior and Systematics
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351236/
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLOS Genetics
publication_identifier:
  issn:
  - 1553-7404
publication_status: published
publisher: Public Library of Science (PLoS)
quality_controlled: '1'
scopus_import: '1'
status: public
title: AXR1 affects DNA methylation independently of its role in regulating meiotic
  crossover localization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...