Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1

article Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1 published yes Michael Prattes author Irina Grishkovskaya author Victor-Valentin Hodirnau author 3661B498-F248-11E8-B48F-1D18A9856A87 Christina Hetzmannseder author Gertrude Zisser author Carolin Sailer author Vasileios Kargas author Mathias Loibl author Magdalena Gerhalter author Lisa Kofler author Alan J. Warren author Florian Stengel author David Haselbach author Helmut Bergler author EM-Fac department The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases. https://research-explorer.ista.ac.at/download/12262/12447/2022_NatureStrucMolecBio_Prattes.pdf application/pdfno Springer Nature2022 eng Molecular BiologyStructural Biology Nature Structural & Molecular Biology 1545-9993 1545-9985 36097293 00085294210000410.1038/s41594-022-00832-5 299942-953 Prattes, Michael, Irina Grishkovskaya, Victor-Valentin Hodirnau, Christina Hetzmannseder, Gertrude Zisser, Carolin Sailer, Vasileios Kargas, et al. “Visualizing Maturation Factor Extraction from the Nascent Ribosome by the AAA-ATPase Drg1.” Nature Structural & Molecular Biology. Springer Nature, 2022. <a href="https://doi.org/10.1038/s41594-022-00832-5">https://doi.org/10.1038/s41594-022-00832-5</a>. Prattes, M., Grishkovskaya, I., Hodirnau, V.-V., Hetzmannseder, C., Zisser, G., Sailer, C., … Bergler, H. (2022). Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1. Nature Structural & Molecular Biology. Springer Nature. <a href="https://doi.org/10.1038/s41594-022-00832-5">https://doi.org/10.1038/s41594-022-00832-5</a> M. Prattes, I. Grishkovskaya, V.-V. Hodirnau, C. Hetzmannseder, G. Zisser, C. Sailer, V. Kargas, M. Loibl, M. Gerhalter, L. Kofler, A.J. Warren, F. Stengel, D. Haselbach, H. Bergler, Nature Structural & Molecular Biology 29 (2022) 942–953. Prattes M, Grishkovskaya I, Hodirnau V-V, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H. 2022. Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1. Nature Structural & Molecular Biology. 29(9), 942–953. Prattes M, Grishkovskaya I, Hodirnau V-V, et al. Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1. Nature Structural & Molecular Biology. 2022;29(9):942-953. doi:<a href="https://doi.org/10.1038/s41594-022-00832-5">10.1038/s41594-022-00832-5</a> Prattes, Michael, et al. “Visualizing Maturation Factor Extraction from the Nascent Ribosome by the AAA-ATPase Drg1.” Nature Structural & Molecular Biology, vol. 29, no. 9, Springer Nature, 2022, pp. 942–53, doi:<a href="https://doi.org/10.1038/s41594-022-00832-5">10.1038/s41594-022-00832-5</a>. M. Prattes et al., “Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1,” Nature Structural & Molecular Biology, vol. 29, no. 9. Springer Nature, pp. 942–953, 2022. 122622023-01-16T09:59:06Z2023-08-04T09:52:20Z