---
_id: '12275'
abstract:
- lang: eng
  text: N-glycans are molecularly diverse sugars borne by over 70% of proteins transiting
    the secretory pathway and have been implicated in protein folding, stability,
    and localization. Mutations in genes important for N-glycosylation result in congenital
    disorders of glycosylation that are often associated with intellectual disability.
    Here, we show that structurally distinct N-glycans regulate an extracellular protein
    complex involved in the patterning of somatosensory dendrites in Caenorhabditis
    elegans. Specifically, aman-2/Golgi alpha-mannosidase II, a conserved key enzyme
    in the biosynthesis of specific N-glycans, regulates the activity of the Menorin
    adhesion complex without obviously affecting the protein stability and localization
    of its components. AMAN-2 functions cell-autonomously to allow for decoration
    of the neuronal transmembrane receptor DMA-1/LRR-TM with the correct set of high-mannose/hybrid/paucimannose
    N-glycans. Moreover, distinct types of N-glycans on specific N-glycosylation sites
    regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular
    proteins, forms the Menorin adhesion complex. In summary, specific N-glycan structures
    regulate dendrite patterning by coordinating the activity of an extracellular
    adhesion complex, suggesting that the molecular diversity of N-glycans can contribute
    to developmental specificity in the nervous system.
acknowledgement: 'We thank Scott Garforth, Sarah Garrett, Peri Kurshan, Yehuda Salzberg,
  PamelaStanley, Robert Townley, and members of the B€ulow laboratory for commentson
  the manuscript or helpful discussions during the course of this work. Wethank David
  Miller, Shohei Mitani, Kang Shen, and Iain Wilson for reagents,and Yuji Kohara for
  theyk11g705cDNA clone. We are grateful to MeeraTrivedi for sharing thedzIs117strain
  prior to publication. Some strains wereprovided by the Caenorhabditis Genome Center
  (funded by the NIH Office ofResearch Infrastructure Programs P40OD010440). This
  work was supportedby grants from the National Institute of Health (NIH): R01NS096672andR21NS111145to
  HEB; F31NS100370to MR; T32GM007288and F31HD066967to CADB; P30HD071593to Albert Einstein
  College of Medicine. We acknowl-edge support to MR by the Department of Neuroscience.
  NJRS was the recipi-ent of a Colciencias-Fulbright Fellowship and HEB of an Irma
  T. Hirschl/Monique Weill-Caulier research fellowship'
article_number: e54163
article_processing_charge: No
article_type: original
author:
- first_name: Maisha
  full_name: Rahman, Maisha
  last_name: Rahman
- first_name: Nelson
  full_name: Ramirez, Nelson
  id: 39831956-E4FE-11E9-85DE-0DC7E5697425
  last_name: Ramirez
- first_name: Carlos A
  full_name: Diaz‐Balzac, Carlos A
  last_name: Diaz‐Balzac
- first_name: Hannes E
  full_name: Bülow, Hannes E
  last_name: Bülow
citation:
  ama: Rahman M, Ramirez N, Diaz‐Balzac CA, Bülow HE. Specific N-glycans regulate
    an extracellular adhesion complex during somatosensory dendrite patterning. <i>EMBO
    Reports</i>. 2022;23(7). doi:<a href="https://doi.org/10.15252/embr.202154163">10.15252/embr.202154163</a>
  apa: Rahman, M., Ramirez, N., Diaz‐Balzac, C. A., &#38; Bülow, H. E. (2022). Specific
    N-glycans regulate an extracellular adhesion complex during somatosensory dendrite
    patterning. <i>EMBO Reports</i>. Embo Press. <a href="https://doi.org/10.15252/embr.202154163">https://doi.org/10.15252/embr.202154163</a>
  chicago: Rahman, Maisha, Nelson Ramirez, Carlos A Diaz‐Balzac, and Hannes E Bülow.
    “Specific N-Glycans Regulate an Extracellular Adhesion Complex during Somatosensory
    Dendrite Patterning.” <i>EMBO Reports</i>. Embo Press, 2022. <a href="https://doi.org/10.15252/embr.202154163">https://doi.org/10.15252/embr.202154163</a>.
  ieee: M. Rahman, N. Ramirez, C. A. Diaz‐Balzac, and H. E. Bülow, “Specific N-glycans
    regulate an extracellular adhesion complex during somatosensory dendrite patterning,”
    <i>EMBO Reports</i>, vol. 23, no. 7. Embo Press, 2022.
  ista: Rahman M, Ramirez N, Diaz‐Balzac CA, Bülow HE. 2022. Specific N-glycans regulate
    an extracellular adhesion complex during somatosensory dendrite patterning. EMBO
    Reports. 23(7), e54163.
  mla: Rahman, Maisha, et al. “Specific N-Glycans Regulate an Extracellular Adhesion
    Complex during Somatosensory Dendrite Patterning.” <i>EMBO Reports</i>, vol. 23,
    no. 7, e54163, Embo Press, 2022, doi:<a href="https://doi.org/10.15252/embr.202154163">10.15252/embr.202154163</a>.
  short: M. Rahman, N. Ramirez, C.A. Diaz‐Balzac, H.E. Bülow, EMBO Reports 23 (2022).
date_created: 2023-01-16T10:01:44Z
date_published: 2022-07-05T00:00:00Z
date_updated: 2026-06-18T17:26:25Z
day: '05'
ddc:
- '570'
department:
- _id: MaDe
doi: 10.15252/embr.202154163
external_id:
  isi:
  - '000797302700001'
  pmid:
  - '35586945'
fulldoi: https://doi.org/10.15252/embr.202154163
has_accepted_license: '1'
intvolume: '        23'
isi: 1
issue: '7'
keyword:
- Genetics
- Molecular Biology
- Biochemistry
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.15252/embr.202154163
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Reports
publication_identifier:
  eissn:
  - 1469-3178
  issn:
  - 1469-221X
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Specific N-glycans regulate an extracellular adhesion complex during somatosensory
  dendrite patterning
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2022'
...