---
res:
  bibo_abstract:
  - Neurons extend axons to form the complex circuitry of the mature brain. This depends
    on the coordinated response and continuous remodelling of the microtubule and
    F-actin networks in the axonal growth cone. Growth cone architecture remains poorly
    understood at nanoscales. We therefore investigated mouse hippocampal neuron growth
    cones using cryo-electron tomography to directly visualise their three-dimensional
    subcellular architecture with molecular detail. Our data showed that the hexagonal
    arrays of actin bundles that form filopodia penetrate and terminate deep within
    the growth cone interior. We directly observed the modulation of these and other
    growth cone actin bundles by alteration of individual F-actin helical structures.
    Microtubules with blunt, slightly flared or gently curved ends predominated in
    the growth cone, frequently contained lumenal particles and exhibited lattice
    defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental
    cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies
    in overall growth cone organisation or in F-actin subpopulations. However, our
    data suggested that microtubules sustained more structural defects, highlighting
    the importance of microtubule integrity during growth cone migration.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Joseph
      foaf_name: Atherton, Joseph
      foaf_surname: Atherton
  - foaf_Person:
      foaf_givenName: Melissa A
      foaf_name: Stouffer, Melissa A
      foaf_surname: Stouffer
      foaf_workInfoHomepage: http://www.librecat.org/personId=4C9372C4-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Fiona
      foaf_name: Francis, Fiona
      foaf_surname: Francis
  - foaf_Person:
      foaf_givenName: Carolyn A.
      foaf_name: Moores, Carolyn A.
      foaf_surname: Moores
  bibo_doi: 10.1242/jcs.259234
  bibo_issue: '7'
  bibo_volume: 135
  dct_date: 2022^xs_gYear
  dct_identifier:
  - UT:000783840400010
  dct_isPartOf:
  - http://id.crossref.org/issn/0021-9533
  - http://id.crossref.org/issn/1477-9137
  dct_language: eng
  dct_publisher: The Company of Biologists@
  dct_subject:
  - Cell Biology
  dct_title: Visualising the cytoskeletal machinery in neuronal growth cones using
    cryo-electron tomography@
  fabio_hasPubmedId: '35383828'
...