---
_id: '12288'
abstract:
- lang: eng
  text: To understand the function of neuronal circuits, it is crucial to disentangle
    the connectivity patterns within the network. However, most tools currently used
    to explore connectivity have low throughput, low selectivity, or limited accessibility.
    Here, we report the development of an improved packaging system for the production
    of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers
    orders of magnitude higher with no background contamination, at a fraction of
    the production time, while preserving the efficiency of transsynaptic labeling.
    Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic
    RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal
    populations, tailored for diverse experimental requirements. We demonstrate the
    power and flexibility of the new system by uncovering hidden local and distal
    inhibitory connections in the mouse hippocampal formation and by imaging the functional
    properties of a cortical microcircuit across weeks. Our novel production pipeline
    provides a convenient approach to generate new rabies vectors, while our toolkit
    flexibly and efficiently expands the current capacity to label, manipulate and
    image the neuronal activity of interconnected neuronal circuits in vitro and in
    vivo.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c
  viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript.
  This research was supported by the Scientific Service Units (SSU) of IST-Austria
  through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical
  Facility (PCF). This project was funded by the European Research Council (ERC) under
  the European Union’s Horizon 2020 research and innovation programme (ERC advanced
  grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der
  Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier
  Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).
article_number: '79848'
article_processing_charge: No
article_type: original
author:
- first_name: Anton L
  full_name: Sumser, Anton L
  id: 3320A096-F248-11E8-B48F-1D18A9856A87
  last_name: Sumser
  orcid: 0000-0002-4792-1881
- first_name: Maximilian A
  full_name: Jösch, Maximilian A
  id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
  last_name: Jösch
  orcid: 0000-0002-3937-1330
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
- first_name: Yoav
  full_name: Ben Simon, Yoav
  id: 43DF3136-F248-11E8-B48F-1D18A9856A87
  last_name: Ben Simon
citation:
  ama: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production
    of improved rabies viral vectors for specific, efficient and versatile transsynaptic
    retrograde labeling. <i>eLife</i>. 2022;11. doi:<a href="https://doi.org/10.7554/elife.79848">10.7554/elife.79848</a>
  apa: Sumser, A. L., Jösch, M. A., Jonas, P. M., &#38; Ben Simon, Y. (2022). Fast,
    high-throughput production of improved rabies viral vectors for specific, efficient
    and versatile transsynaptic retrograde labeling. <i>ELife</i>. eLife Sciences
    Publications. <a href="https://doi.org/10.7554/elife.79848">https://doi.org/10.7554/elife.79848</a>
  chicago: Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon.
    “Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific,
    Efficient and Versatile Transsynaptic Retrograde Labeling.” <i>ELife</i>. eLife
    Sciences Publications, 2022. <a href="https://doi.org/10.7554/elife.79848">https://doi.org/10.7554/elife.79848</a>.
  ieee: A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput
    production of improved rabies viral vectors for specific, efficient and versatile
    transsynaptic retrograde labeling,” <i>eLife</i>, vol. 11. eLife Sciences Publications,
    2022.
  ista: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production
    of improved rabies viral vectors for specific, efficient and versatile transsynaptic
    retrograde labeling. eLife. 11, 79848.
  mla: Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies
    Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.”
    <i>ELife</i>, vol. 11, 79848, eLife Sciences Publications, 2022, doi:<a href="https://doi.org/10.7554/elife.79848">10.7554/elife.79848</a>.
  short: A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).
corr_author: '1'
date_created: 2023-01-16T10:04:15Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2025-04-15T08:29:05Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PeJo
doi: 10.7554/elife.79848
ec_funded: 1
external_id:
  isi:
  - '000892204300001'
  pmid:
  - '36040301'
file:
- access_level: open_access
  checksum: 5a2a65e3e7225090c3d8199f3bbd7b7b
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-30T11:50:53Z
  date_updated: 2023-01-30T11:50:53Z
  file_id: '12463'
  file_name: 2022_eLife_Sumser.pdf
  file_size: 8506811
  relation: main_file
  success: 1
file_date_updated: 2023-01-30T11:50:53Z
has_accepted_license: '1'
intvolume: '        11'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '756502'
  name: Circuits of Visual Attention
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: Synaptic communication in neuronal microcircuits
- _id: 266D407A-B435-11E9-9278-68D0E5697425
  grant_number: LT000256
  name: Neuronal networks of salience and spatial detection in the murine superior
    colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 1098-2017
  name: Connecting sensory with motor processing in the superior colliculus
publication: eLife
publication_identifier:
  eissn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fast, high-throughput production of improved rabies viral vectors for specific,
  efficient and versatile transsynaptic retrograde labeling
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2022'
...