article published yes Jiří Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 Michelle C Gallei author 35A03822-F248-11E8-B48F-1D18A9856A870000-0003-1286-7368 Zuzana Gelová author 0AE74790-0E0B-11E9-ABC7-1ACFE56974250000-0003-4783-1752 Alexander J Johnson author 46A62C3A-F248-11E8-B48F-1D18A9856A870000-0002-2739-8843 Ewa Mazur author Aline Monzer author 2DB5D88C-D7B3-11E9-B8FD-7907E6697425 Lesia Rodriguez Solovey author 3922B506-F248-11E8-B48F-1D18A9856A870000-0002-7244-7237 Mark Roosjen author Inge Verstraeten author 362BF7FE-F248-11E8-B48F-1D18A9856A870000-0001-7241-2328 Branka D. Živanović author Minxia Zou author 5c243f41-03f3-11ec-841c-96faf48a7ef9 Lukas Fiedler author 7c417475-8972-11ed-ae7b-8b674ca26986 Caterina Giannini author e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 Peter Grones author Mónika Hrtyan author 45A71A74-F248-11E8-B48F-1D18A9856A87 Walter Kaufmann author 3F99E422-F248-11E8-B48F-1D18A9856A870000-0001-9735-5315 Andre Kuhn author Madhumitha Narasimhan author 44BF24D0-F248-11E8-B48F-1D18A9856A870000-0002-8600-0671 Marek Randuch author 6ac4636d-15b2-11ec-abd3-fb8df79972ae Nikola Rýdza author Koji Takahashi author Shutang Tan author 2DE75584-F248-11E8-B48F-1D18A9856A870000-0002-0471-8285 Anastasiia Teplova author e3736151-106c-11ec-b916-c2558e2762c6 Toshinori Kinoshita author Dolf Weijers author Hana Rakusová author JiFr department GradSch department EvBe department EM-Fac department Tracing Evolution of Auxin Transport and Polarity in Plants project RNA-directed DNA methylation in plant development project The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization. https://research-explorer.ista.ac.at/download/12291/14483/Friml Nature 2022_merged.pdf application/pdfno Springer Nature2022 eng 0028-0836 1476-4687 36071161 00085135750000210.1038/s41586-022-05187-x 6097927575-581 https://research-explorer.ista.ac.at/record/19395 https://research-explorer.ista.ac.at/record/20364 Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581. J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581. J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022. Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href="https://doi.org/10.1038/s41586-022-05187-x">10.1038/s41586-022-05187-x</a> Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href="https://doi.org/10.1038/s41586-022-05187-x">10.1038/s41586-022-05187-x</a>. Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href="https://doi.org/10.1038/s41586-022-05187-x">https://doi.org/10.1038/s41586-022-05187-x</a>. Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href="https://doi.org/10.1038/s41586-022-05187-x">https://doi.org/10.1038/s41586-022-05187-x</a> 122912023-01-16T10:04:48Z2026-04-07T11:52:15Z