---
res:
  bibo_abstract:
  - Together, copy-number and point mutations form the basis for most evolutionary
    novelty, through the process of gene duplication and divergence. While a plethora
    of genomic data reveals the long-term fate of diverging coding sequences and their
    cis-regulatory elements, little is known about the early dynamics around the duplication
    event itself. In microorganisms, selection for increased gene expression often
    drives the expansion of gene copy-number mutations, which serves as a crude adaptation,
    prior to divergence through refining point mutations. Using a simple synthetic
    genetic reporter system that can distinguish between copy-number and point mutations,
    we study their early and transient adaptive dynamics in real time in Escherichia
    coli. We find two qualitatively different routes of adaptation, depending on the
    level of functional improvement needed. In conditions of high gene expression
    demand, the two mutation types occur as a combination. However, under low gene
    expression demand, copy-number and point mutations are mutually exclusive; here,
    owing to their higher frequency, adaptation is dominated by copy-number mutations,
    in a process we term amplification hindrance. Ultimately, due to high reversal
    rates and pleiotropic cost, copy-number mutations may not only serve as a crude
    and transient adaptation, but also constrain sequence divergence over evolutionary
    time scales.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Isabella
      foaf_name: Tomanek, Isabella
      foaf_surname: Tomanek
      foaf_workInfoHomepage: http://www.librecat.org/personId=3981F020-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6197-363X
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Guet, Calin C
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  bibo_doi: 10.7554/ELIFE.82240
  bibo_volume: 11
  dct_date: 2022^xs_gYear
  dct_identifier:
  - UT:000912674700001
  dct_isPartOf:
  - http://id.crossref.org/issn/2050-084X
  dct_language: eng
  dct_publisher: eLife Sciences Publications@
  dct_title: Adaptation dynamics between copynumber and point mutations@
  fabio_hasPubmedId: '36546673'
...