---
res:
  bibo_abstract:
  - 'Copy-number and point mutations form the basis for most evolutionary novelty
    through the process of gene duplication and divergence. While a plethora of genomic
    sequence data reveals the long-term fate of diverging coding sequences and their
    cis-regulatory elements, little is known about the early dynamics around the duplication
    event itself. In microorganisms, selection for increased gene expression often
    drives the expansion of gene copy-number mutations, which serves as a crude adaptation,
    prior to divergence through refining point mutations. Using a simple synthetic
    genetic system that allows us to distinguish copy-number and point mutations,
    we study their early and transient adaptive dynamics in real-time in Escherichia
    coli. We find two qualitatively different routes of adaptation depending on the
    level of functional improvement selected for: In conditions of high gene expression
    demand, the two types of mutations occur as a combination. Under low gene expression
    demand, negative epistasis between the two types of mutations renders them mutually
    exclusive. Thus, owing to their higher frequency, adaptation is dominated by copy-number
    mutations. Ultimately, due to high rates of reversal and pleiotropic cost, copy-number
    mutations may not only serve as a crude and transient adaptation but also constrain
    sequence divergence over evolutionary time scales.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Isabella
      foaf_name: Tomanek, Isabella
      foaf_surname: Tomanek
      foaf_workInfoHomepage: http://www.librecat.org/personId=3981F020-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6197-363X
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Guet, Calin C
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  bibo_doi: 10.5061/dryad.rfj6q57ds
  dct_date: 2022^xs_gYear
  dct_publisher: Dryad@
  dct_title: Flow cytometry YFP and CFP data and deep sequencing data of populations
    evolving in galactose@
...