---
_id: '12339'
abstract:
- lang: eng
  text: 'Copy-number and point mutations form the basis for most evolutionary novelty
    through the process of gene duplication and divergence. While a plethora of genomic
    sequence data reveals the long-term fate of diverging coding sequences and their
    cis-regulatory elements, little is known about the early dynamics around the duplication
    event itself. In microorganisms, selection for increased gene expression often
    drives the expansion of gene copy-number mutations, which serves as a crude adaptation,
    prior to divergence through refining point mutations. Using a simple synthetic
    genetic system that allows us to distinguish copy-number and point mutations,
    we study their early and transient adaptive dynamics in real-time in Escherichia
    coli. We find two qualitatively different routes of adaptation depending on the
    level of functional improvement selected for: In conditions of high gene expression
    demand, the two types of mutations occur as a combination. Under low gene expression
    demand, negative epistasis between the two types of mutations renders them mutually
    exclusive. Thus, owing to their higher frequency, adaptation is dominated by copy-number
    mutations. Ultimately, due to high rates of reversal and pleiotropic cost, copy-number
    mutations may not only serve as a crude and transient adaptation but also constrain
    sequence divergence over evolutionary time scales.'
article_processing_charge: No
author:
- first_name: Isabella
  full_name: Tomanek, Isabella
  id: 3981F020-F248-11E8-B48F-1D18A9856A87
  last_name: Tomanek
  orcid: 0000-0001-6197-363X
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
citation:
  ama: Tomanek I, Guet CC. Flow cytometry YFP and CFP data and deep sequencing data
    of populations evolving in galactose. 2022. doi:<a href="https://doi.org/10.5061/dryad.rfj6q57ds">10.5061/dryad.rfj6q57ds</a>
  apa: Tomanek, I., &#38; Guet, C. C. (2022). Flow cytometry YFP and CFP data and
    deep sequencing data of populations evolving in galactose. Dryad. <a href="https://doi.org/10.5061/dryad.rfj6q57ds">https://doi.org/10.5061/dryad.rfj6q57ds</a>
  chicago: Tomanek, Isabella, and Calin C Guet. “Flow Cytometry YFP and CFP Data and
    Deep Sequencing Data of Populations Evolving in Galactose.” Dryad, 2022. <a href="https://doi.org/10.5061/dryad.rfj6q57ds">https://doi.org/10.5061/dryad.rfj6q57ds</a>.
  ieee: I. Tomanek and C. C. Guet, “Flow cytometry YFP and CFP data and deep sequencing
    data of populations evolving in galactose.” Dryad, 2022.
  ista: Tomanek I, Guet CC. 2022. Flow cytometry YFP and CFP data and deep sequencing
    data of populations evolving in galactose, Dryad, <a href="https://doi.org/10.5061/dryad.rfj6q57ds">10.5061/dryad.rfj6q57ds</a>.
  mla: Tomanek, Isabella, and Calin C. Guet. <i>Flow Cytometry YFP and CFP Data and
    Deep Sequencing Data of Populations Evolving in Galactose</i>. Dryad, 2022, doi:<a
    href="https://doi.org/10.5061/dryad.rfj6q57ds">10.5061/dryad.rfj6q57ds</a>.
  short: I. Tomanek, C.C. Guet, (2022).
corr_author: '1'
date_created: 2023-01-23T09:00:37Z
date_published: 2022-12-23T00:00:00Z
date_updated: 2025-03-06T14:03:50Z
day: '23'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.5061/dryad.rfj6q57ds
main_file_link:
- open_access: '1'
  url: https://doi.org/10.5061/dryad.rfj6q57ds
month: '12'
oa: 1
oa_version: Published Version
publisher: Dryad
related_material:
  record:
  - id: '12333'
    relation: used_in_publication
    status: public
status: public
title: Flow cytometry YFP and CFP data and deep sequencing data of populations evolving
  in galactose
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2022'
...