thesis published Gloria Colombo author 3483CF6C-F248-11E8-B48F-1D18A9856A870000-0001-9434-8902 Sandra Siegert supervisor GradSch department SaSi department International IST Doctoral Program project Environmental cues influence the highly dynamic morphology of microglia. Strategies to characterize these changes usually involve user-selected morphometric features, which preclude the identification of a spectrum of context-dependent morphological phenotypes. Here, we develop MorphOMICs, a topological data analysis approach, which enables semiautomatic mapping of microglial morphology into an atlas of cue-dependent phenotypes, overcomes feature-selection bias and minimizes biological variability. First, with MorphOMICs we derive the morphological spectrum of microglia across seven brain regions during postnatal development and in two distinct Alzheimer’s disease degeneration mouse models. We uncover region-specific and sexually dimorphic morphological trajectories, with females showing an earlier morphological shift than males in the degenerating brain. Overall, we demonstrate that both long primary- and short terminal processes provide distinct insights to morphological phenotypes. Moreover, using machine learning to map novel condition on the spectrum, we observe that microglia morphologies reflect a dose-dependent adaptation upon ketamine anesthesia and do not recover to control morphologies. Next, we took advantage of MorphOMICs to build a high-resolution and layer-specific map of microglial morphological spectrum in the retina, covering postnatal development and rd10 degeneration. Here, following photoreceptor death, microglia assume an early developmentlike morphology. Finally, we map microglial morphology following optic nerve crush on the retinal spectrum and observe a layer- and sex-dependent response. Overall, MorphOMICs opens a new perspective to analyze microglial morphology across multiple conditions, and provides a novel tool to characterize microglial morphology beyond the traditionally dichotomized view of microglia. https://research-explorer.ista.ac.at/download/12378/12379/Gloria_Colombo_Thesis.docx application/vnd.openxmlformats-officedocument.wordprocessingml.document https://research-explorer.ista.ac.at/download/12378/12380/Gloria_Colombo_Thesis.pdf application/pdf2023-04-11no Institute of Science and Technology Austria2022 eng 2663-337X10.15479/at:ista:12378 142 https://research-explorer.ista.ac.at/record/12244 Colombo, G. (2022). <i>MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/at:ista:12378">https://doi.org/10.15479/at:ista:12378</a> Colombo, Gloria. <i>MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes</i>. Institute of Science and Technology Austria, 2022, doi:<a href="https://doi.org/10.15479/at:ista:12378">10.15479/at:ista:12378</a>. Colombo, Gloria. “MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes.” Institute of Science and Technology Austria, 2022. <a href="https://doi.org/10.15479/at:ista:12378">https://doi.org/10.15479/at:ista:12378</a>. Colombo G. MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes. 2022. doi:<a href="https://doi.org/10.15479/at:ista:12378">10.15479/at:ista:12378</a> Colombo G. 2022. MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes. Institute of Science and Technology Austria. G. Colombo, “MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes,” Institute of Science and Technology Austria, 2022. G. Colombo, MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes, Institute of Science and Technology Austria, 2022. 123782023-01-25T14:27:43Z2026-04-07T14:29:41Z