---
_id: '12478'
abstract:
- lang: eng
  text: In Gram negative bacteria, the multiple antibiotic resistance or mar operon,
    is known to control the expression of multi-drug efflux genes that protect bacteria
    from a wide range of drugs. As many different chemical compounds can induce this
    operon, identifying the parameters that govern the dynamics of its induction is
    crucial to better characterize the processes of tolerance and resistance. Most
    experiments have assumed that the properties of the mar transcriptional network
    can be inferred from population measurements. However, measurements from an asynchronous
    population of cells can mask underlying phenotypic variations of single cells.
    We monitored the activity of the mar promoter in single Escherichia coli cells
    in linear micro-colonies and established that the response to a steady level of
    inducer was most heterogeneous within individual colonies for an intermediate
    value of inducer. Specifically, sub-lineages defined by contiguous daughter-cells
    exhibited similar promoter activity, whereas activity was greatly variable between
    different sub-lineages. Specific sub-trees of uniform promoter activity persisted
    over several generations. Statistical analyses of the lineages suggest that the
    presence of these sub-trees is the signature of an inducible memory of the promoter
    state that is transmitted from mother to daughter cells. This single-cell study
    reveals that the degree of epigenetic inheritance changes as a function of inducer
    concentration, suggesting that phenotypic inheritance may be an inducible phenotype.
acknowledgement: This work was supported by NIH P50 award P50GM081892-02 to the University
  of Chicago, a catalyst grant from the Chicago Biomedical Consortium with support
  from The Searle Funds at The Chicago Community Trust to PC, and a Yen Fellowship
  to CCG. MA was partially supported by PAPIIT-UNAM grant IN-11322.
article_number: '1049255'
article_processing_charge: Yes
article_type: original
author:
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: L
  full_name: Bruneaux, L
  last_name: Bruneaux
- first_name: P
  full_name: Oikonomou, P
  last_name: Oikonomou
- first_name: M
  full_name: Aldana, M
  last_name: Aldana
- first_name: P
  full_name: Cluzel, P
  last_name: Cluzel
citation:
  ama: Guet CC, Bruneaux L, Oikonomou P, Aldana M, Cluzel P. Monitoring lineages of
    growing and dividing bacteria reveals an inducible memory of <i>mar</i> operon
    expression. <i>Frontiers in Microbiology</i>. 2023;14. doi:<a href="https://doi.org/10.3389/fmicb.2023.1049255">10.3389/fmicb.2023.1049255</a>
  apa: Guet, C. C., Bruneaux, L., Oikonomou, P., Aldana, M., &#38; Cluzel, P. (2023).
    Monitoring lineages of growing and dividing bacteria reveals an inducible memory
    of <i>mar</i> operon expression. <i>Frontiers in Microbiology</i>. Frontiers.
    <a href="https://doi.org/10.3389/fmicb.2023.1049255">https://doi.org/10.3389/fmicb.2023.1049255</a>
  chicago: Guet, Calin C, L Bruneaux, P Oikonomou, M Aldana, and P Cluzel. “Monitoring
    Lineages of Growing and Dividing Bacteria Reveals an Inducible Memory of <i>Mar</i>
    Operon Expression.” <i>Frontiers in Microbiology</i>. Frontiers, 2023. <a href="https://doi.org/10.3389/fmicb.2023.1049255">https://doi.org/10.3389/fmicb.2023.1049255</a>.
  ieee: C. C. Guet, L. Bruneaux, P. Oikonomou, M. Aldana, and P. Cluzel, “Monitoring
    lineages of growing and dividing bacteria reveals an inducible memory of <i>mar</i>
    operon expression,” <i>Frontiers in Microbiology</i>, vol. 14. Frontiers, 2023.
  ista: Guet CC, Bruneaux L, Oikonomou P, Aldana M, Cluzel P. 2023. Monitoring lineages
    of growing and dividing bacteria reveals an inducible memory of <i>mar</i> operon
    expression. Frontiers in Microbiology. 14, 1049255.
  mla: Guet, Calin C., et al. “Monitoring Lineages of Growing and Dividing Bacteria
    Reveals an Inducible Memory of <i>Mar</i> Operon Expression.” <i>Frontiers in
    Microbiology</i>, vol. 14, 1049255, Frontiers, 2023, doi:<a href="https://doi.org/10.3389/fmicb.2023.1049255">10.3389/fmicb.2023.1049255</a>.
  short: C.C. Guet, L. Bruneaux, P. Oikonomou, M. Aldana, P. Cluzel, Frontiers in
    Microbiology 14 (2023).
corr_author: '1'
date_created: 2023-02-02T08:13:28Z
date_published: 2023-06-20T00:00:00Z
date_updated: 2024-10-09T21:03:59Z
day: '20'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.3389/fmicb.2023.1049255
external_id:
  isi:
  - '001030002600001'
  pmid:
  - '37485524'
file:
- access_level: open_access
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  creator: dernst
  date_created: 2023-07-31T07:16:34Z
  date_updated: 2023-07-31T07:16:34Z
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  file_size: 6452841
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file_date_updated: 2023-07-31T07:16:34Z
has_accepted_license: '1'
intvolume: '        14'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Microbiology
publication_identifier:
  eissn:
  - 1664-302X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Monitoring lineages of growing and dividing bacteria reveals an inducible memory
  of <i>mar</i> operon expression
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2023'
...