---
_id: '1250'
abstract:
- lang: eng
  text: In bacteria, replicative aging manifests as a difference in growth or survival
    between the two cells emerging from division. One cell can be regarded as an aging
    mother with a decreased potential for future survival and division, the other
    as a rejuvenated daughter. Here, we aimed at investigating some of the processes
    involved in aging in the bacterium Escherichia coli, where the two types of cells
    can be distinguished by the age of their cell poles. We found that certain changes
    in the regulation of the carbohydrate metabolism can affect aging. A mutation
    in the carbon storage regulator gene, csrA, leads to a dramatically shorter replicative
    lifespan; csrA mutants stop dividing once their pole exceeds an age of about five
    divisions. These old-pole cells accumulate glycogen at their old cell poles; after
    their last division, they do not contain a chromosome, presumably because of spatial
    exclusion by the glycogen aggregates. The new-pole daughters produced by these
    aging mothers are born young; they only express the deleterious phenotype once
    their pole is old. These results demonstrate how manipulations of nutrient allocation
    can lead to the exclusion of the chromosome and limit replicative lifespan in
    E. coli, and illustrate how mutations can have phenotypic effects that are specific
    for cells with old poles. This raises the question how bacteria can avoid the
    accumulation of such mutations in their genomes over evolutionary times, and how
    they can achieve the long replicative lifespans that have recently been reported.
acknowledgement: This manuscript is dedicated to the memory of Alex Böhm, who was
  a great friend and a passionate biologist. Alex passed away after the initial submission
  of this manuscript. We thank Vesna Olivera and Ursula Sauder from the Zentrum für
  Mikroskopie Uni Basel for excellent service, and Olin Silander, Nikki Freed, and
  Nela Nikolic for helpful discussions. This work was supported by the Swiss National
  Science Foundation grants to M. Ackermann and Urs Jenal (supporting AB).
article_number: e1005974
article_processing_charge: No
author:
- first_name: Alex
  full_name: Boehm, Alex
  last_name: Boehm
- first_name: Markus
  full_name: Arnoldini, Markus
  last_name: Arnoldini
- first_name: Tobias
  full_name: Bergmiller, Tobias
  id: 2C471CFA-F248-11E8-B48F-1D18A9856A87
  last_name: Bergmiller
  orcid: 0000-0001-5396-4346
- first_name: Thomas
  full_name: Röösli, Thomas
  last_name: Röösli
- first_name: Colette
  full_name: Bigosch, Colette
  last_name: Bigosch
- first_name: Martin
  full_name: Ackermann, Martin
  last_name: Ackermann
citation:
  ama: Boehm A, Arnoldini M, Bergmiller T, Röösli T, Bigosch C, Ackermann M. Genetic
    manipulation of glycogen allocation affects replicative lifespan in E coli. <i>PLoS
    Genetics</i>. 2016;12(4). doi:<a href="https://doi.org/10.1371/journal.pgen.1005974">10.1371/journal.pgen.1005974</a>
  apa: Boehm, A., Arnoldini, M., Bergmiller, T., Röösli, T., Bigosch, C., &#38; Ackermann,
    M. (2016). Genetic manipulation of glycogen allocation affects replicative lifespan
    in E coli. <i>PLoS Genetics</i>. Public Library of Science. <a href="https://doi.org/10.1371/journal.pgen.1005974">https://doi.org/10.1371/journal.pgen.1005974</a>
  chicago: Boehm, Alex, Markus Arnoldini, Tobias Bergmiller, Thomas Röösli, Colette
    Bigosch, and Martin Ackermann. “Genetic Manipulation of Glycogen Allocation Affects
    Replicative Lifespan in E Coli.” <i>PLoS Genetics</i>. Public Library of Science,
    2016. <a href="https://doi.org/10.1371/journal.pgen.1005974">https://doi.org/10.1371/journal.pgen.1005974</a>.
  ieee: A. Boehm, M. Arnoldini, T. Bergmiller, T. Röösli, C. Bigosch, and M. Ackermann,
    “Genetic manipulation of glycogen allocation affects replicative lifespan in E
    coli,” <i>PLoS Genetics</i>, vol. 12, no. 4. Public Library of Science, 2016.
  ista: Boehm A, Arnoldini M, Bergmiller T, Röösli T, Bigosch C, Ackermann M. 2016.
    Genetic manipulation of glycogen allocation affects replicative lifespan in E
    coli. PLoS Genetics. 12(4), e1005974.
  mla: Boehm, Alex, et al. “Genetic Manipulation of Glycogen Allocation Affects Replicative
    Lifespan in E Coli.” <i>PLoS Genetics</i>, vol. 12, no. 4, e1005974, Public Library
    of Science, 2016, doi:<a href="https://doi.org/10.1371/journal.pgen.1005974">10.1371/journal.pgen.1005974</a>.
  short: A. Boehm, M. Arnoldini, T. Bergmiller, T. Röösli, C. Bigosch, M. Ackermann,
    PLoS Genetics 12 (2016).
date_created: 2018-12-11T11:50:56Z
date_published: 2016-04-19T00:00:00Z
date_updated: 2025-09-22T09:10:03Z
day: '19'
ddc:
- '576'
- '579'
department:
- _id: CaGu
doi: 10.1371/journal.pgen.1005974
external_id:
  isi:
  - '000375231900025'
file:
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file_date_updated: 2020-07-14T12:44:41Z
has_accepted_license: '1'
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language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
publication: PLoS Genetics
publication_status: published
publisher: Public Library of Science
publist_id: '6077'
pubrep_id: '705'
quality_controlled: '1'
related_material:
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    status: public
scopus_import: '1'
status: public
title: Genetic manipulation of glycogen allocation affects replicative lifespan in
  E coli
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 12
year: '2016'
...