---
_id: '1251'
abstract:
- lang: eng
  text: Plant growth and architecture is regulated by the polar distribution of the
    hormone auxin. Polarity and flexibility of this process is provided by constant
    cycling of auxin transporter vesicles along actin filaments, coordinated by a
    positive auxinactin feedback loop. Both polar auxin transport and vesicle cycling
    are inhibited by synthetic auxin transport inhibitors, such as 1-Nnaphthylphthalamic
    acid (NPA), counteracting the effect of auxin; however, underlying targets and
    mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis
    thaliana ABCB chaperone TWISTED DWARF1 (TWD1).We identify ACTIN7 as a relevant,
    although likely indirect, TWD1 interactor, and show TWD1-dependent regulation
    of actin filament organization and dynamics and that TWD1 is required for NPA-mediated
    actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence
    of efflux transporters, and as a consequence act7 and twd1 share developmental
    and physiological phenotypes indicative of defects in auxin transport. These can
    be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide
    evidence that TWD1 determines downstreamlocations of auxin efflux transporters
    by adjusting actin filament debundling and dynamizing processes and mediating
    NPA action on the latter. This function appears to be evolutionary conserved since
    TWD1 expression in budding yeast alters actin polarization and cell polarity and
    provides NPA sensitivity.
acknowledgement: ' This work was supported by grants from the European Social Fund
  (CZ.1.07/2.3.00/20.0043), the Czech Science Foundation GAČR (GA13-40637S) to J.F.
  and M.Z., the Ministry of Education, Youth, and Sports of the Czech Republic under
  the project CEITEC 2020 (LQ1601) to M.Z., the Ministry for Higher Education and
  Research of Luxembourg (REC-LOCM-20140703) to C.T., the Partial Funding Program
  for Short Stays Abroad of CONICET Argentina (to N.I.B.), Swiss National Funds, the
  Pool de Recherche of the University of Fribourg, and the Novartis Foundation (all
  to M.G.). '
article_processing_charge: No
author:
- first_name: Jinsheng
  full_name: Zhu, Jinsheng
  last_name: Zhu
- first_name: Aurélien
  full_name: Bailly, Aurélien
  last_name: Bailly
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Valpuri
  full_name: Sovero, Valpuri
  last_name: Sovero
- first_name: Martin
  full_name: Di Donato, Martin
  last_name: Di Donato
- first_name: Pei
  full_name: Ge, Pei
  last_name: Ge
- first_name: Jacqueline
  full_name: Oehri, Jacqueline
  last_name: Oehri
- first_name: Bibek
  full_name: Aryal, Bibek
  last_name: Aryal
- first_name: Pengchao
  full_name: Hao, Pengchao
  last_name: Hao
- first_name: Miriam
  full_name: Linnert, Miriam
  last_name: Linnert
- first_name: Noelia
  full_name: Burgardt, Noelia
  last_name: Burgardt
- first_name: Christian
  full_name: Lücke, Christian
  last_name: Lücke
- first_name: Matthias
  full_name: Weiwad, Matthias
  last_name: Weiwad
- first_name: Max
  full_name: Michel, Max
  last_name: Michel
- first_name: Oliver
  full_name: Weiergräber, Oliver
  last_name: Weiergräber
- first_name: Stephan
  full_name: Pollmann, Stephan
  last_name: Pollmann
- first_name: Elisa
  full_name: Azzarello, Elisa
  last_name: Azzarello
- first_name: Stefano
  full_name: Mancuso, Stefano
  last_name: Mancuso
- first_name: Noel
  full_name: Ferro, Noel
  last_name: Ferro
- first_name: Yoichiro
  full_name: Fukao, Yoichiro
  last_name: Fukao
- first_name: Céline
  full_name: Hoffmann, Céline
  last_name: Hoffmann
- first_name: Roland
  full_name: Wedlich Söldner, Roland
  last_name: Wedlich Söldner
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Clément
  full_name: Thomas, Clément
  last_name: Thomas
- first_name: Markus
  full_name: Geisler, Markus
  last_name: Geisler
citation:
  ama: Zhu J, Bailly A, Zwiewka M, et al. TWISTED DWARF1 mediates the action of auxin
    transport inhibitors on actin cytoskeleton dynamics. <i>Plant Cell</i>. 2016;28(4):930-948.
    doi:<a href="https://doi.org/10.1105/tpc.15.00726">10.1105/tpc.15.00726</a>
  apa: Zhu, J., Bailly, A., Zwiewka, M., Sovero, V., Di Donato, M., Ge, P., … Geisler,
    M. (2016). TWISTED DWARF1 mediates the action of auxin transport inhibitors on
    actin cytoskeleton dynamics. <i>Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.15.00726">https://doi.org/10.1105/tpc.15.00726</a>
  chicago: Zhu, Jinsheng, Aurélien Bailly, Marta Zwiewka, Valpuri Sovero, Martin Di
    Donato, Pei Ge, Jacqueline Oehri, et al. “TWISTED DWARF1 Mediates the Action of
    Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.” <i>Plant Cell</i>.
    American Society of Plant Biologists, 2016. <a href="https://doi.org/10.1105/tpc.15.00726">https://doi.org/10.1105/tpc.15.00726</a>.
  ieee: J. Zhu <i>et al.</i>, “TWISTED DWARF1 mediates the action of auxin transport
    inhibitors on actin cytoskeleton dynamics,” <i>Plant Cell</i>, vol. 28, no. 4.
    American Society of Plant Biologists, pp. 930–948, 2016.
  ista: Zhu J, Bailly A, Zwiewka M, Sovero V, Di Donato M, Ge P, Oehri J, Aryal B,
    Hao P, Linnert M, Burgardt N, Lücke C, Weiwad M, Michel M, Weiergräber O, Pollmann
    S, Azzarello E, Mancuso S, Ferro N, Fukao Y, Hoffmann C, Wedlich Söldner R, Friml
    J, Thomas C, Geisler M. 2016. TWISTED DWARF1 mediates the action of auxin transport
    inhibitors on actin cytoskeleton dynamics. Plant Cell. 28(4), 930–948.
  mla: Zhu, Jinsheng, et al. “TWISTED DWARF1 Mediates the Action of Auxin Transport
    Inhibitors on Actin Cytoskeleton Dynamics.” <i>Plant Cell</i>, vol. 28, no. 4,
    American Society of Plant Biologists, 2016, pp. 930–48, doi:<a href="https://doi.org/10.1105/tpc.15.00726">10.1105/tpc.15.00726</a>.
  short: J. Zhu, A. Bailly, M. Zwiewka, V. Sovero, M. Di Donato, P. Ge, J. Oehri,
    B. Aryal, P. Hao, M. Linnert, N. Burgardt, C. Lücke, M. Weiwad, M. Michel, O.
    Weiergräber, S. Pollmann, E. Azzarello, S. Mancuso, N. Ferro, Y. Fukao, C. Hoffmann,
    R. Wedlich Söldner, J. Friml, C. Thomas, M. Geisler, Plant Cell 28 (2016) 930–948.
date_created: 2018-12-11T11:50:57Z
date_published: 2016-04-01T00:00:00Z
date_updated: 2025-09-22T09:10:52Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.15.00726
external_id:
  isi:
  - '000375416000012'
intvolume: '        28'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863381/
month: '04'
oa: 1
oa_version: Submitted Version
page: 930 - 948
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6078'
quality_controlled: '1'
scopus_import: '1'
status: public
title: TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton
  dynamics
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 28
year: '2016'
...