--- res: bibo_abstract: - Cytosine methylation within CG dinucleotides (mCG) can be epigenetically inherited over many generations. Such inheritance is thought to be mediated by a semiconservative mechanism that produces binary present/absent methylation patterns. However, we show here that in Arabidopsis thaliana h1ddm1 mutants, intermediate heterochromatic mCG is stably inherited across many generations and is quantitatively associated with transposon expression. We develop a mathematical model that estimates the rates of semiconservative maintenance failure and de novo methylation at each transposon, demonstrating that mCG can be stably inherited at any level via a dynamic balance of these activities. We find that DRM2 – the core methyltransferase of the RNA-directed DNA methylation pathway – catalyzes most of the heterochromatic de novo mCG, with de novo rates orders of magnitude higher than previously thought, whereas chromomethylases make smaller contributions. Our results demonstrate that stable epigenetic inheritance of mCG in plant heterochromatin is enabled by extensive de novo methylation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: David B. foaf_name: Lyons, David B. foaf_surname: Lyons - foaf_Person: foaf_givenName: Amy foaf_name: Briffa, Amy foaf_surname: Briffa - foaf_Person: foaf_givenName: Shengbo foaf_name: He, Shengbo foaf_surname: He - foaf_Person: foaf_givenName: Jaemyung foaf_name: Choi, Jaemyung foaf_surname: Choi - foaf_Person: foaf_givenName: Elizabeth foaf_name: Hollwey, Elizabeth foaf_surname: Hollwey foaf_workInfoHomepage: http://www.librecat.org/personId=b8c4f54b-e484-11eb-8fdc-a54df64ef6dd - foaf_Person: foaf_givenName: Jack foaf_name: Colicchio, Jack foaf_surname: Colicchio - foaf_Person: foaf_givenName: Ian foaf_name: Anderson, Ian foaf_surname: Anderson - foaf_Person: foaf_givenName: Xiaoqi foaf_name: Feng, Xiaoqi foaf_surname: Feng foaf_workInfoHomepage: http://www.librecat.org/personId=e0164712-22ee-11ed-b12a-d80fcdf35958 orcid: 0000-0002-4008-1234 - foaf_Person: foaf_givenName: Martin foaf_name: Howard, Martin foaf_surname: Howard - foaf_Person: foaf_givenName: Daniel foaf_name: Zilberman, Daniel foaf_surname: Zilberman foaf_workInfoHomepage: http://www.librecat.org/personId=6973db13-dd5f-11ea-814e-b3e5455e9ed1 orcid: 0000-0002-0123-8649 bibo_doi: 10.1016/j.celrep.2023.112132 bibo_issue: '3' bibo_volume: 42 dct_date: 2023^xs_gYear dct_identifier: - UT:000944921600001 dct_isPartOf: - http://id.crossref.org/issn/2211-1247 dct_language: eng dct_publisher: Elsevier@ dct_title: Extensive de novo activity stabilizes epigenetic inheritance of CG methylation in Arabidopsis transposons@ ...