{"publication":"Angewandte Chemie International Edition","doi":"10.1002/anie.202219314","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"isi":["000956919900001"],"pmid":["36738230"]},"quality_controlled":"1","license":"https://creativecommons.org/licenses/by-nc/4.0/","issue":"19","title":"The rigid core and flexible surface of amyloid fibrils probed by Magic‐Angle Spinning NMR of aromatic residues","article_type":"original","oa":1,"related_material":{"link":[{"url":"https://ista.ac.at/en/news/dancing-styles-of-atoms/","relation":"press_release","description":"News on ISTA website"}],"record":[{"id":"14861","status":"public","relation":"other"},{"id":"12497","status":"public","relation":"research_data"}]},"keyword":["General Chemistry","Catalysis"],"publication_identifier":{"eissn":["1521-3773"],"issn":["1433-7851"]},"language":[{"iso":"eng"}],"article_number":"e202219314","pmid":1,"_id":"12675","acknowledgement":"We thank AlbertA. Smith (Leipzig)for insightful discussions. This work was supported by funding from the European Research Council (StG-2012-311318 to P.S.) and used the platforms of the Grenoble Instruct-ERIC center (ISBG;UMS 3518 CNRS-CEA-UJF-EMBL) within the Grenoble Partnership for Structural Biology(PSB) and facilities and expertiseof the Biophysical and Structural Chemistry platform (BPCS) at IECB,CNRSUAR3033,INSERMUS001 and Bordeaux University.","intvolume":"        62","file_date_updated":"2023-08-16T12:33:31Z","oa_version":"Published Version","day":"01","volume":62,"publisher":"Wiley","file":[{"file_name":"2023_AngewChemInt_Becker.pdf","file_id":"14072","creator":"dernst","date_created":"2023-08-16T12:33:31Z","relation":"main_file","checksum":"7dd083ed8850faa55c34e411ed390de9","date_updated":"2023-08-16T12:33:31Z","access_level":"open_access","success":1,"file_size":1422445,"content_type":"application/pdf"}],"ddc":["540"],"date_updated":"2024-02-21T12:14:06Z","month":"05","publication_status":"published","tmp":{"short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)"},"abstract":[{"lang":"eng","text":"Aromatic side chains are important reporters of the plasticity of proteins, and often form important contacts in protein--protein interactions. By studying a pair of structurally homologous cross-β amyloid fibrils, HET-s and HELLF, with a specific isotope-labeling approach and magic-angle-spinning (MAS) NMR, we have characterized the dynamic behavior of Phe and Tyr aromatic rings to show that the hydrophobic amyloid core is rigid, without any sign of \"breathing motions\" over hundreds of milliseconds at least. Aromatic residues exposed at the fibril surface have a rigid ring axis but undergo ring flips, on a variety of time scales from ns to µs. Our approach provides direct insight into hydrophobic-core motions, enabling a better evaluation of the conformational heterogeneity generated from a NMR structural ensemble of such amyloid cross-β architecture."}],"date_published":"2023-05-01T00:00:00Z","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","isi":1,"department":[{"_id":"GradSch"},{"_id":"PaSc"}],"status":"public","author":[{"first_name":"Lea Marie","last_name":"Becker","id":"36336939-eb97-11eb-a6c2-c83f1214ca79","full_name":"Becker, Lea Marie","orcid":"0000-0002-6401-5151"},{"first_name":"Mélanie","last_name":"Berbon","full_name":"Berbon, Mélanie"},{"full_name":"Vallet, Alicia","last_name":"Vallet","first_name":"Alicia"},{"last_name":"Grelard","full_name":"Grelard, Axelle","first_name":"Axelle"},{"last_name":"Morvan","full_name":"Morvan, Estelle","first_name":"Estelle"},{"full_name":"Bardiaux, Benjamin","last_name":"Bardiaux","first_name":"Benjamin"},{"first_name":"Roman","last_name":"Lichtenecker","full_name":"Lichtenecker, Roman"},{"full_name":"Ernst, Matthias","last_name":"Ernst","first_name":"Matthias"},{"first_name":"Antoine","last_name":"Loquet","full_name":"Loquet, Antoine"},{"first_name":"Paul","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","orcid":"0000-0002-9350-7606","last_name":"Schanda"}],"citation":{"ieee":"L. M. Becker <i>et al.</i>, “The rigid core and flexible surface of amyloid fibrils probed by Magic‐Angle Spinning NMR of aromatic residues,” <i>Angewandte Chemie International Edition</i>, vol. 62, no. 19. Wiley, 2023.","ama":"Becker LM, Berbon M, Vallet A, et al. The rigid core and flexible surface of amyloid fibrils probed by Magic‐Angle Spinning NMR of aromatic residues. <i>Angewandte Chemie International Edition</i>. 2023;62(19). doi:<a href=\"https://doi.org/10.1002/anie.202219314\">10.1002/anie.202219314</a>","mla":"Becker, Lea Marie, et al. “The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle Spinning NMR of Aromatic Residues.” <i>Angewandte Chemie International Edition</i>, vol. 62, no. 19, e202219314, Wiley, 2023, doi:<a href=\"https://doi.org/10.1002/anie.202219314\">10.1002/anie.202219314</a>.","ista":"Becker LM, Berbon M, Vallet A, Grelard A, Morvan E, Bardiaux B, Lichtenecker R, Ernst M, Loquet A, Schanda P. 2023. The rigid core and flexible surface of amyloid fibrils probed by Magic‐Angle Spinning NMR of aromatic residues. Angewandte Chemie International Edition. 62(19), e202219314.","chicago":"Becker, Lea Marie, Mélanie Berbon, Alicia Vallet, Axelle Grelard, Estelle Morvan, Benjamin Bardiaux, Roman Lichtenecker, Matthias Ernst, Antoine Loquet, and Paul Schanda. “The Rigid Core and Flexible Surface of Amyloid Fibrils Probed by Magic‐Angle Spinning NMR of Aromatic Residues.” <i>Angewandte Chemie International Edition</i>. Wiley, 2023. <a href=\"https://doi.org/10.1002/anie.202219314\">https://doi.org/10.1002/anie.202219314</a>.","apa":"Becker, L. M., Berbon, M., Vallet, A., Grelard, A., Morvan, E., Bardiaux, B., … Schanda, P. (2023). The rigid core and flexible surface of amyloid fibrils probed by Magic‐Angle Spinning NMR of aromatic residues. <i>Angewandte Chemie International Edition</i>. Wiley. <a href=\"https://doi.org/10.1002/anie.202219314\">https://doi.org/10.1002/anie.202219314</a>","short":"L.M. Becker, M. Berbon, A. Vallet, A. Grelard, E. Morvan, B. Bardiaux, R. Lichtenecker, M. Ernst, A. Loquet, P. Schanda, Angewandte Chemie International Edition 62 (2023)."},"type":"journal_article","year":"2023","date_created":"2023-02-24T10:45:01Z"}