article published yes Giselle T Cheung author 471195F6-F248-11E8-B48F-1D18A9856A870000-0001-8457-2572 Florian Pauler author 48EA0138-F248-11E8-B48F-1D18A9856A870000-0002-7462-0048 Peter Koppensteiner author 3B8B25A8-F248-11E8-B48F-1D18A9856A870000-0002-3509-1948 Thomas Krausgruber author Carmen Streicher author 36BCB99C-F248-11E8-B48F-1D18A9856A87 Martin Schrammel author f13e7cae-e8bd-11ed-841a-96dedf69f46d Natalie Y Özgen author e68ece33-f6e0-11ea-865d-ae1031dcc090 Alexis Ivec author 1d144691-e8be-11ed-9b33-bdd3077fad4c Christoph Bock author Ryuichi Shigemoto author 499F3ABC-F248-11E8-B48F-1D18A9856A870000-0001-8761-9444 Simon Hippenmeyer author 37B36620-F248-11E8-B48F-1D18A9856A870000-0003-2279-1061 SiHi department RySh department Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression project The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. However, the developmental principles directing the generation of SC cell-type diversity are not understood. Here, we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at the single-RGP/-cell level of the mammalian SC ontogeny. https://research-explorer.ista.ac.at/download/12875/14944/2024_Neuron_Cheung.pdf application/pdfno Elsevier2024 eng 0896-6273 38096816 00116393790000110.1016/j.neuron.2023.11.009 1122230-246.e11 https://ista.ac.at/en/news/the-pedigree-of-brain-cells/ G.T. Cheung, F. Pauler, P. Koppensteiner, T. Krausgruber, C. Streicher, M. Schrammel, N.Y. Özgen, A. Ivec, C. Bock, R. Shigemoto, S. Hippenmeyer, Neuron 112 (2024) 230–246.e11. Cheung, Giselle T., et al. “Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus.” <i>Neuron</i>, vol. 112, no. 2, Elsevier, 2024, p. 230–246.e11, doi:<a href="https://doi.org/10.1016/j.neuron.2023.11.009">10.1016/j.neuron.2023.11.009</a>. Cheung GT, Pauler F, Koppensteiner P, Krausgruber T, Streicher C, Schrammel M, Özgen NY, Ivec A, Bock C, Shigemoto R, Hippenmeyer S. 2024. Multipotent progenitors instruct ontogeny of the superior colliculus. Neuron. 112(2), 230–246.e11. Cheung GT, Pauler F, Koppensteiner P, et al. Multipotent progenitors instruct ontogeny of the superior colliculus. <i>Neuron</i>. 2024;112(2):230-246.e11. doi:<a href="https://doi.org/10.1016/j.neuron.2023.11.009">10.1016/j.neuron.2023.11.009</a> Cheung, Giselle T, Florian Pauler, Peter Koppensteiner, Thomas Krausgruber, Carmen Streicher, Martin Schrammel, Natalie Y Özgen, et al. “Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus.” <i>Neuron</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.neuron.2023.11.009">https://doi.org/10.1016/j.neuron.2023.11.009</a>. G. T. Cheung <i>et al.</i>, “Multipotent progenitors instruct ontogeny of the superior colliculus,” <i>Neuron</i>, vol. 112, no. 2. Elsevier, p. 230–246.e11, 2024. Cheung, G. T., Pauler, F., Koppensteiner, P., Krausgruber, T., Streicher, C., Schrammel, M., … Hippenmeyer, S. (2024). Multipotent progenitors instruct ontogeny of the superior colliculus. <i>Neuron</i>. Elsevier. <a href="https://doi.org/10.1016/j.neuron.2023.11.009">https://doi.org/10.1016/j.neuron.2023.11.009</a> 128752023-04-27T09:41:48Z2025-12-30T10:54:12Z