---
res:
  bibo_abstract:
  - "The tight spatiotemporal coordination of signaling activity determining embryo\r\npatterning
    and the physical processes driving embryo morphogenesis renders\r\nembryonic development
    robust, such that key developmental processes can unfold\r\nrelatively normally
    even outside of the full embryonic context. For instance, embryonic\r\nstem cell
    cultures can recapitulate the hallmarks of gastrulation, i.e. break symmetry\r\nleading
    to germ layer formation and morphogenesis, in a very reduced environment.\r\nThis
    leads to questions on specific contributions of embryo-specific features, such
    as\r\nthe presence of extraembryonic tissues, which are inherently involved in
    gastrulation\r\nin the full embryonic context. To address this, we established
    zebrafish embryonic\r\nexplants without the extraembryonic yolk cell, an important
    player as a signaling\r\nsource and for morphogenesis during gastrulation, as
    a model of ex vivo development.\r\nWe found that dorsal-marginal determinants
    are required and sufficient in these\r\nexplants to form and pattern all three
    germ layers. However, formation of tissues,\r\nwhich require the highest Nodal-signaling
    levels, is variable, demonstrating a\r\ncontribution of extraembryonic tissues
    for reaching peak Nodal signaling levels.\r\nBlastoderm explants also undergo
    gastrulation-like axis elongation. We found that this\r\nelongation movement shows
    hallmarks of oriented mesendoderm cell intercalations\r\ntypically associated
    with dorsal tissues in the intact embryo. These are disrupted by\r\nuniform upregulation
    of BMP signaling activity and concomitant explant ventralization,\r\nsuggesting
    that tight spatial control of BMP signaling is a prerequisite for explant\r\nmorphogenesis.
    This control is achieved by Nodal signaling, which is critical for\r\neffectively
    downregulating BMP signaling in the mesendoderm, highlighting that Nodal\r\nsignaling
    is not only directly required for mesendoderm cell fate specification and\r\nmorphogenesis,
    but also by maintaining low levels of BMP signaling at the dorsal side.\r\nCollectively,
    we provide insights into the capacity and organization of signaling and\r\nmorphogenetic
    domains to recapitulate features of zebrafish gastrulation outside of\r\nthe full
    embryonic context.@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Alexandra
      foaf_name: Schauer, Alexandra
      foaf_surname: Schauer
      foaf_workInfoHomepage: http://www.librecat.org/personId=30A536BA-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7659-9142
  bibo_doi: 10.15479/at:ista:12891
  dct_date: 2023^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2663-337X
  dct_language: eng
  dct_publisher: Institute of Science and Technology Austria@
  dct_title: 'Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic
    tissues@'
...