---
res:
  bibo_abstract:
  - Genetically encoded fluorescent probes of neural activity represent new promising
    tools for systems neuroscience. Here, we present a comparative in vivo analysis
    of 10 different genetically encoded calcium indicators, as well as the pH-sensitive
    synapto-pHluorin. We analyzed their fluorescence changes in presynaptic boutons
    of the Drosophila larval neuromuscular junction. Robust neural activity did not
    result in any or noteworthy fluorescence changes when Flash-Pericam, Camgaroo-1,
    and Camgaroo-2 were expressed. However, calculated on the raw data, fractional
    fluorescence changes up to 18% were reported by synapto-pHluorin, Yellow Cameleon
    2.0, 2.3, and 3.3, Inverse-Pericam, GCaMP1.3, GCaMP1.6, and the troponin C-based
    calcium sensor TN-L15. The response characteristics of all of these indicators
    differed considerably from each other, with GCaMP1.6 reporting high rates of neural
    activity with the largest and fastest fluorescence changes. However, GCaMP1.6
    suffered from photobleaching, whereas the fluorescence signals of the double-chromophore
    indicators were in general smaller but more photostable and reproducible, with
    TN-L15 showing the fastest rise of the signals at lower activity rates. We show
    for GCaMP1.3 and YC3.3 that an expanded range of neural activity evoked fairly
    linear fluorescence changes and a corresponding linear increase in the signal-to-noise
    ratio (SNR). The expression level of the indicator biased the signal kinetics
    and SNR, whereas the signal amplitude was independent. The presented data will
    be useful for in vivo experiments with respect to the selection of an appropriate
    indicator, as well as for the correct interpretation of the optical signals.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Dierk
      foaf_name: Reiff, Dierk
      foaf_surname: Reiff
  - foaf_Person:
      foaf_givenName: Alexandra
      foaf_name: Ihring, Alexandra
      foaf_surname: Ihring
  - foaf_Person:
      foaf_givenName: Giovanna
      foaf_name: Guerrero, Giovanna
      foaf_surname: Guerrero
  - foaf_Person:
      foaf_givenName: Ehud
      foaf_name: Isacoff, Ehud
      foaf_surname: Isacoff
  - foaf_Person:
      foaf_givenName: Maximilian A
      foaf_name: Jösch, Maximilian A
      foaf_surname: Jösch
      foaf_workInfoHomepage: http://www.librecat.org/personId=2BD278E6-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-3937-1330
  - foaf_Person:
      foaf_givenName: Junichi
      foaf_name: Nakai, Junichi
      foaf_surname: Nakai
  - foaf_Person:
      foaf_givenName: Alexander
      foaf_name: Borst, Alexander
      foaf_surname: Borst
  bibo_doi: 10.1523/JNEUROSCI.4900-04.2005
  bibo_issue: '19'
  bibo_volume: 25
  dct_date: 2005^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0270-6474
  - http://id.crossref.org/issn/1529-2401
  dct_language: eng
  dct_publisher: Society for Neuroscience@
  dct_title: In vivo performance of genetically encoded indicators of neural activity
    in flies@
  fabio_hasPubmedId: '15888652'
...