{"has_accepted_license":"1","file":[{"relation":"source_file","checksum":"74f3f89e59a0189bee53ebfad9c1b9af","file_id":"13089","date_created":"2023-05-25T06:32:12Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_name":"Thesis_final_LauraBocanegra.docx","file_size":25615534,"access_level":"closed","creator":"lbocaneg","date_updated":"2023-05-25T06:32:12Z"},{"file_size":12386046,"access_level":"closed","embargo_to":"open_access","creator":"lbocaneg","date_updated":"2023-05-25T06:32:16Z","checksum":"c6cdef6323eacfb4b7a8af20f32eae97","relation":"main_file","file_id":"13090","date_created":"2023-05-25T06:32:16Z","content_type":"application/pdf","embargo":"2024-05-31","file_name":"TotalFinal_Thesis_LauraBocanegraArx.pdf"}],"author":[{"id":"4896F754-F248-11E8-B48F-1D18A9856A87","first_name":"Laura","full_name":"Bocanegra, Laura","last_name":"Bocanegra"}],"_id":"13081","year":"2023","publication_identifier":{"issn":["2663 - 337X"]},"supervisor":[{"id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","last_name":"Kicheva","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","first_name":"Anna"}],"ddc":["570"],"date_updated":"2023-10-04T11:14:04Z","doi":"10.15479/at:ista:13081","department":[{"_id":"GradSch"},{"_id":"AnKi"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"day":"23","citation":{"ieee":"L. Bocanegra, “Epithelial dynamics during mouse neural tube development,” Institute of Science and Technology Austria, 2023.","ama":"Bocanegra L. Epithelial dynamics during mouse neural tube development. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:13081\">10.15479/at:ista:13081</a>","apa":"Bocanegra, L. (2023). <i>Epithelial dynamics during mouse neural tube development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:13081\">https://doi.org/10.15479/at:ista:13081</a>","short":"L. Bocanegra, Epithelial Dynamics during Mouse Neural Tube Development, Institute of Science and Technology Austria, 2023.","mla":"Bocanegra, Laura. <i>Epithelial Dynamics during Mouse Neural Tube Development</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:13081\">10.15479/at:ista:13081</a>.","ista":"Bocanegra L. 2023. Epithelial dynamics during mouse neural tube development. Institute of Science and Technology Austria.","chicago":"Bocanegra, Laura. “Epithelial Dynamics during Mouse Neural Tube Development.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:13081\">https://doi.org/10.15479/at:ista:13081</a>."},"abstract":[{"text":"During development, tissues undergo changes in size and shape to form functional organs. Distinct cellular processes such as cell division and cell rearrangements underlie tissue morphogenesis. Yet how the distinct processes are controlled and coordinated, and how they contribute to morphogenesis is poorly understood. In our study, we addressed these questions using the developing mouse neural tube. This epithelial organ transforms from a flat epithelial sheet to an epithelial tube while increasing in size and undergoing morpho-gen-mediated patterning. The extent and mechanism of neural progenitor rearrangement within the developing mouse neuroepithelium is unknown. To investigate this, we per-formed high resolution lineage tracing analysis to quantify the extent of epithelial rear-rangement at different stages of neural tube development. We quantitatively described the relationship between apical cell size with cell cycle dependent interkinetic nuclear migra-tions (IKNM) and performed high cellular resolution live imaging of the neuroepithelium to study the dynamics of junctional remodeling.  Furthermore, developed a vertex model of the neuroepithelium to investigate the quantitative contribution of cell proliferation, cell differentiation and mechanical properties to the epithelial rearrangement dynamics and validated the model predictions through functional experiments. Our analysis revealed that at early developmental stages, the apical cell area kinetics driven by IKNM induce high lev-els of cell rearrangements in a regime of high junctional tension and contractility. After E9.5, there is a sharp decline in the extent of cell rearrangements, suggesting that the epi-thelium transitions from a fluid-like to a solid-like state. We found that this transition is regulated by the growth rate of the tissue, rather than by changes in cell-cell adhesion and contractile forces. Overall, our study provides a quantitative description of the relationship between tissue growth, cell cycle dynamics, epithelia rearrangements and the emergent tissue material properties, and novel insights on how epithelial cell dynamics influences tissue morphogenesis.","lang":"eng"}],"article_processing_charge":"No","status":"public","file_date_updated":"2023-05-25T06:32:16Z","publication_status":"published","page":"93","degree_awarded":"PhD","type":"dissertation","publisher":"Institute of Science and Technology Austria","date_published":"2023-05-23T00:00:00Z","date_created":"2023-05-23T19:10:42Z","oa_version":"Published Version","related_material":{"record":[{"id":"9349","relation":"part_of_dissertation","status":"public"},{"id":"12837","status":"public","relation":"part_of_dissertation"}]},"language":[{"iso":"eng"}],"alternative_title":["ISTA Thesis"],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"05","title":"Epithelial dynamics during mouse neural tube development"}