{"intvolume":"       145","author":[{"full_name":"Troussicot, Laura","first_name":"Laura","last_name":"Troussicot","id":"3d9cac31-413c-11eb-9514-d1ec2a7fb7f3"},{"last_name":"Vallet","first_name":"Alicia","full_name":"Vallet, Alicia"},{"full_name":"Molin, Mikael","first_name":"Mikael","last_name":"Molin"},{"first_name":"Björn M.","full_name":"Burmann, Björn M.","last_name":"Burmann"},{"orcid":"0000-0002-9350-7606","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","full_name":"Schanda, Paul","first_name":"Paul","last_name":"Schanda"}],"article_processing_charge":"No","oa":1,"oa_version":"Published Version","issue":"19","publication_identifier":{"issn":["0002-7863"],"eissn":["1520-5126"]},"abstract":[{"lang":"eng","text":"Disulfide bond formation is fundamentally important for protein structure and constitutes a key mechanism by which cells regulate the intracellular oxidation state. Peroxiredoxins (PRDXs) eliminate reactive oxygen species such as hydrogen peroxide through a catalytic cycle of Cys oxidation and reduction. Additionally, upon Cys oxidation PRDXs undergo extensive conformational rearrangements that may underlie their presently structurally poorly defined functions as molecular chaperones. Rearrangements include high molecular-weight oligomerization, the dynamics of which are, however, poorly understood, as is the impact of disulfide bond formation on these properties. Here we show that formation of disulfide bonds along the catalytic cycle induces extensive μs time scale dynamics, as monitored by magic-angle spinning NMR of the 216 kDa-large Tsa1 decameric assembly and solution-NMR of a designed dimeric mutant. We ascribe the conformational dynamics to structural frustration, resulting from conflicts between the disulfide-constrained reduction of mobility and the desire to fulfill other favorable contacts."}],"date_published":"2023-05-04T00:00:00Z","date_updated":"2023-08-01T14:48:09Z","doi":"10.1021/jacs.3c01200","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"PaSc"}],"volume":145,"status":"public","article_type":"original","_id":"13095","publication":"Journal of the American Chemical Society","external_id":{"pmid":["37140345"],"isi":["000985907400001"]},"type":"journal_article","scopus_import":"1","day":"04","isi":1,"month":"05","language":[{"iso":"eng"}],"publication_status":"published","quality_controlled":"1","acknowledgement":"We thank Albert A. Smith (Univ. Leipzig) for discussions and help with detectors analyses, Undina Guillerm (IST Austria) for gel electrophoresis experiments (Figure S7), and Jens\r\nLidman (Univ. Gothenburg) for a 3Q relaxation analysis script. Intramural funding from Institute of Science and Technology Austria is acknowledged. This work also used the platforms of\r\nthe Grenoble Instruct-ERIC center (ISBG; UMS 3518 CNRSCEA-UJF-EMBL) within the Grenoble Partnership for Structural Biology (PSB), as well as the Swedish NMR Centre\r\nof the University of Gothenburg. Both platforms provided excellent research infrastructures. B.M.B. gratefully acknowledges funding from the Swedish Research Council (Starting grant 2016-04721), the Swedish Cancer Foundation (2019-0415), and the Knut och Alice Wallenberg Foundation through a Wallenberg Academy Fellowship (2016.0163) as well as through the Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Sweden. ","publisher":"American Chemical Society","date_created":"2023-05-28T22:01:04Z","page":"10700–10711","has_accepted_license":"1","title":"Disulfide-bond-induced structural frustration and dynamic disorder in a peroxiredoxin from MAS NMR","file":[{"file_name":"2023_JACS_Troussicot.pdf","creator":"dernst","success":1,"relation":"main_file","checksum":"0758a930ef21c62fc91b14e657479f83","date_created":"2023-05-30T07:05:28Z","access_level":"open_access","date_updated":"2023-05-30T07:05:28Z","file_id":"13098","file_size":6719299,"content_type":"application/pdf"}],"ddc":["540"],"pmid":1,"year":"2023","related_material":{"record":[{"id":"12820","status":"public","relation":"research_data"}]},"file_date_updated":"2023-05-30T07:05:28Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"citation":{"ieee":"L. Troussicot, A. Vallet, M. Molin, B. M. Burmann, and P. Schanda, “Disulfide-bond-induced structural frustration and dynamic disorder in a peroxiredoxin from MAS NMR,” <i>Journal of the American Chemical Society</i>, vol. 145, no. 19. American Chemical Society, pp. 10700–10711, 2023.","apa":"Troussicot, L., Vallet, A., Molin, M., Burmann, B. M., &#38; Schanda, P. (2023). Disulfide-bond-induced structural frustration and dynamic disorder in a peroxiredoxin from MAS NMR. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.3c01200\">https://doi.org/10.1021/jacs.3c01200</a>","chicago":"Troussicot, Laura, Alicia Vallet, Mikael Molin, Björn M. Burmann, and Paul Schanda. “Disulfide-Bond-Induced Structural Frustration and Dynamic Disorder in a Peroxiredoxin from MAS NMR.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2023. <a href=\"https://doi.org/10.1021/jacs.3c01200\">https://doi.org/10.1021/jacs.3c01200</a>.","ista":"Troussicot L, Vallet A, Molin M, Burmann BM, Schanda P. 2023. Disulfide-bond-induced structural frustration and dynamic disorder in a peroxiredoxin from MAS NMR. Journal of the American Chemical Society. 145(19), 10700–10711.","short":"L. Troussicot, A. Vallet, M. Molin, B.M. Burmann, P. Schanda, Journal of the American Chemical Society 145 (2023) 10700–10711.","ama":"Troussicot L, Vallet A, Molin M, Burmann BM, Schanda P. Disulfide-bond-induced structural frustration and dynamic disorder in a peroxiredoxin from MAS NMR. <i>Journal of the American Chemical Society</i>. 2023;145(19):10700–10711. doi:<a href=\"https://doi.org/10.1021/jacs.3c01200\">10.1021/jacs.3c01200</a>","mla":"Troussicot, Laura, et al. “Disulfide-Bond-Induced Structural Frustration and Dynamic Disorder in a Peroxiredoxin from MAS NMR.” <i>Journal of the American Chemical Society</i>, vol. 145, no. 19, American Chemical Society, 2023, pp. 10700–10711, doi:<a href=\"https://doi.org/10.1021/jacs.3c01200\">10.1021/jacs.3c01200</a>."}}