{"abstract":[{"text":"Ustilago maydis is a biotrophic phytopathogenic fungus that causes corn smut disease. As a well-established model system, U. maydis is genetically fully accessible with large omics datasets available and subject to various biological questions ranging from DNA-repair, RNA-transport, and protein secretion to disease biology. For many genetic approaches, tight control of transgene regulation is important. Here we established an optimised version of the Tetracycline-ON (TetON) system for U. maydis. We demonstrate the Tetracycline concentration-dependent expression of fluorescent protein transgenes and the system’s suitability for the induced expression of the toxic protein BCL2 Associated X-1 (Bax1). The Golden Gate compatible vector system contains a native minimal promoter from the mating factor a-1 encoding gene, mfa with ten copies of the tet-regulated operator (tetO) and a codon optimised Tet-repressor (tetR*) which is translationally fused to the native transcriptional corepressor Mql1 (UMAG_05501). The metabolism-independent transcriptional regulator system is functional both, in liquid culture as well as on solid media in the presence of the inducer and can become a useful tool for toxin-antitoxin studies, identification of antifungal proteins, and to study functions of toxic gene products in Ustilago maydis.","lang":"eng"}],"quality_controlled":"1","file_date_updated":"2023-07-17T11:46:34Z","year":"2022","department":[{"_id":"JiFr"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","volume":3,"oa":1,"date_updated":"2024-03-06T14:01:57Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"acknowledgement":"The research leading to these results received funding from the European Research Council under the European Union’s Seventh Framework Programme ERC-2013-STG (grant agreement: 335691), the Austrian Science Fund (I 3033-B22), the Austrian Academy of Sciences, and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy EXC-2070-390732324 (PhenoRob) and DFG grant (DJ 64/5-1).\r\nWe would like to thank the GMI/IMBA/IMP core facilities for their excellent technical support. We would like to acknowledge Dr. Sinéad A. O’Sullivan from DZNE, University of Bonn for providing anti-GFP antibodies. The authors are thankful to the Excellence University of Bonn for providing infrastructure and instrumentation facilities at the INRES-Plant Pathology department.","title":"Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis","intvolume":"         3","type":"journal_article","article_type":"original","citation":{"short":"K.D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, A. Djamei, Frontiers in Fungal Biology 3 (2022).","ista":"Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. 2022. Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis. Frontiers in Fungal Biology. 3, 1029114.","chicago":"Ingole, Kishor D., Nithya Nagarajan, Simon Uhse, Caterina Giannini, and Armin Djamei. “Tetracycline-Controlled (TetON) Gene Expression System for the Smut Fungus Ustilago Maydis.” <i>Frontiers in Fungal Biology</i>. Frontiers Media, 2022. <a href=\"https://doi.org/10.3389/ffunb.2022.1029114\">https://doi.org/10.3389/ffunb.2022.1029114</a>.","mla":"Ingole, Kishor D., et al. “Tetracycline-Controlled (TetON) Gene Expression System for the Smut Fungus Ustilago Maydis.” <i>Frontiers in Fungal Biology</i>, vol. 3, 1029114, Frontiers Media, 2022, doi:<a href=\"https://doi.org/10.3389/ffunb.2022.1029114\">10.3389/ffunb.2022.1029114</a>.","ieee":"K. D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, and A. Djamei, “Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis,” <i>Frontiers in Fungal Biology</i>, vol. 3. Frontiers Media, 2022.","apa":"Ingole, K. D., Nagarajan, N., Uhse, S., Giannini, C., &#38; Djamei, A. (2022). Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis. <i>Frontiers in Fungal Biology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/ffunb.2022.1029114\">https://doi.org/10.3389/ffunb.2022.1029114</a>","ama":"Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis. <i>Frontiers in Fungal Biology</i>. 2022;3. doi:<a href=\"https://doi.org/10.3389/ffunb.2022.1029114\">10.3389/ffunb.2022.1029114</a>"},"doi":"10.3389/ffunb.2022.1029114","file":[{"success":1,"access_level":"open_access","checksum":"2254e0119c0749d6f7237084fefcece6","creator":"dernst","file_size":27966699,"file_id":"13242","file_name":"2023_FrontiersFungalBio_Ingole.pdf","relation":"main_file","date_updated":"2023-07-17T11:46:34Z","date_created":"2023-07-17T11:46:34Z","content_type":"application/pdf"}],"publisher":"Frontiers Media","author":[{"last_name":"Ingole","first_name":"Kishor D.","full_name":"Ingole, Kishor D."},{"last_name":"Nagarajan","first_name":"Nithya","full_name":"Nagarajan, Nithya"},{"last_name":"Uhse","full_name":"Uhse, Simon","first_name":"Simon"},{"last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina","first_name":"Caterina"},{"last_name":"Djamei","first_name":"Armin","full_name":"Djamei, Armin"}],"license":"https://creativecommons.org/licenses/by/4.0/","oa_version":"Published Version","article_number":"1029114","publication_identifier":{"eissn":["2673-6128"]},"month":"10","article_processing_charge":"Yes","has_accepted_license":"1","date_published":"2022-10-19T00:00:00Z","publication_status":"published","_id":"13240","publication":"Frontiers in Fungal Biology","day":"19","status":"public","date_created":"2023-07-16T22:01:12Z","ddc":["579"],"scopus_import":"1","language":[{"iso":"eng"}]}