article published yes Ann K Huylmans author 4C0A3874-F248-11E8-B48F-1D18A9856A870000-0001-8871-4961 Alberto López Ezquerra author John Parsch author Mathilde Cordellier author BeVi department Daphnia species have become models for ecological genomics and exhibit interesting features, such as high phenotypic plasticity and a densely packed genome with many lineage-specific genes. They are also cyclic parthenogenetic, with alternating asexual and sexual cycles and environmental sex determination. Here, we present a de novo transcriptome assembly of over 32,000 D. galeata genes and use it to investigate gene expression in females and spontaneously produced males of two clonal lines derived from lakes in Germany and the Czech Republic. We find that only a low percentage (18%) of genes shows sex-biased expression and that there are many more female-biased gene (FBG) than male-biased gene (MBG). Furthermore, FBGs tend to be more conserved between species than MBGs in both sequence and expression. These patterns may be a consequence of cyclic parthenogenesis leading to a relaxation of purifying selection on MBGs. The two clonal lines show considerable differences in both number and identity of sex-biased genes, suggesting that they may have reproductive strategies differing in their investment in sexual reproduction. Orthologs of key genes in the sex determination and juvenile hormone pathways, which are thought to be important for the transition from asexual to sexual reproduction, are present in D. galeata and highly conserved among Daphnia species. https://research-explorer.ista.ac.at/download/1329/4924/IST-2016-663-v1+1_Genome_Biol_Evol-2016-Huylmans-3120-39.pdf application/pdfno Oxford University Press2016 eng 00038612130000510.1093/gbe/evw221 8103120 - 3139 Huylmans AK, López Ezquerra A, Parsch J, Cordellier M. De novo transcriptome assembly and sex-biased gene expression in the cyclical parthenogenetic Daphnia galeata. <i>Genome Biology and Evolution</i>. 2016;8(10):3120-3139. doi:<a href="https://doi.org/10.1093/gbe/evw221">10.1093/gbe/evw221</a> Huylmans, Ann K., et al. “De Novo Transcriptome Assembly and Sex-Biased Gene Expression in the Cyclical Parthenogenetic Daphnia Galeata.” <i>Genome Biology and Evolution</i>, vol. 8, no. 10, Oxford University Press, 2016, pp. 3120–39, doi:<a href="https://doi.org/10.1093/gbe/evw221">10.1093/gbe/evw221</a>. Huylmans, Ann K, Alberto López Ezquerra, John Parsch, and Mathilde Cordellier. “De Novo Transcriptome Assembly and Sex-Biased Gene Expression in the Cyclical Parthenogenetic Daphnia Galeata.” <i>Genome Biology and Evolution</i>. Oxford University Press, 2016. <a href="https://doi.org/10.1093/gbe/evw221">https://doi.org/10.1093/gbe/evw221</a>. A.K. Huylmans, A. López Ezquerra, J. Parsch, M. Cordellier, Genome Biology and Evolution 8 (2016) 3120–3139. Huylmans AK, López Ezquerra A, Parsch J, Cordellier M. 2016. De novo transcriptome assembly and sex-biased gene expression in the cyclical parthenogenetic Daphnia galeata. Genome Biology and Evolution. 8(10), 3120–3139. Huylmans, A. K., López Ezquerra, A., Parsch, J., &#38; Cordellier, M. (2016). De novo transcriptome assembly and sex-biased gene expression in the cyclical parthenogenetic Daphnia galeata. <i>Genome Biology and Evolution</i>. Oxford University Press. <a href="https://doi.org/10.1093/gbe/evw221">https://doi.org/10.1093/gbe/evw221</a> A. K. Huylmans, A. López Ezquerra, J. Parsch, and M. Cordellier, “De novo transcriptome assembly and sex-biased gene expression in the cyclical parthenogenetic Daphnia galeata,” <i>Genome Biology and Evolution</i>, vol. 8, no. 10. Oxford University Press, pp. 3120–3139, 2016. 13292018-12-11T11:51:24Z2025-09-22T08:24:02Z