{"acknowledgement":"This work was financially supported by the following: The Alabama Agricultural Experiment Station HATCH grants 370222-310010-2055 and 370225-310006-2055 for funding to P.J.Z., E.A.K, A.M.P., and A.M.R. P.J.Z. and E.A.K were supported by an Auburn University Cellular and Molecular Biosciences Research Fellowship. I.D.C. is a postdoctoral fellow of the Research Foundation Flanders (FWO) (FWO/PDO14/043) and is also supported by FWO travel\r\ngrant 12N2415N. F.V.B. was supported by grants from the Interuniversity Attraction Poles Programme (IUAP P7/29 MARS) initiated by the Belgian Science Policy Office and Ghent University (Multidisciplinary Research Partnership Biotechnology for a Sustainable Economy, grant 01MRB510W).","date_updated":"2022-05-24T09:26:03Z","month":"10","title":"Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress","scopus_import":"1","article_processing_charge":"No","date_created":"2018-12-11T11:51:25Z","type":"journal_article","citation":{"chicago":"Zwack, Paul, Inge De Clercq, Timothy Howton, H Tucker Hallmark, Andrej Hurny, Erika Keshishian, Alyssa Parish, et al. “Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1104/pp.16.00415\">https://doi.org/10.1104/pp.16.00415</a>.","apa":"Zwack, P., De Clercq, I., Howton, T., Hallmark, H. T., Hurny, A., Keshishian, E., … Rashotte, A. (2016). Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.00415\">https://doi.org/10.1104/pp.16.00415</a>","ista":"Zwack P, De Clercq I, Howton T, Hallmark HT, Hurny A, Keshishian E, Parish A, Benková E, Mukhtar MS, Van Breusegem F, Rashotte A. 2016. Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress. Plant Physiology. 172(2), 1249–1258.","short":"P. Zwack, I. De Clercq, T. Howton, H.T. Hallmark, A. Hurny, E. Keshishian, A. Parish, E. Benková, M.S. Mukhtar, F. Van Breusegem, A. Rashotte, Plant Physiology 172 (2016) 1249–1258.","ama":"Zwack P, De Clercq I, Howton T, et al. Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress. <i>Plant Physiology</i>. 2016;172(2):1249-1258. doi:<a href=\"https://doi.org/10.1104/pp.16.00415\">10.1104/pp.16.00415</a>","ieee":"P. Zwack <i>et al.</i>, “Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress,” <i>Plant Physiology</i>, vol. 172, no. 2. American Society of Plant Biologists, pp. 1249–1258, 2016.","mla":"Zwack, Paul, et al. “Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.” <i>Plant Physiology</i>, vol. 172, no. 2, American Society of Plant Biologists, 2016, pp. 1249–58, doi:<a href=\"https://doi.org/10.1104/pp.16.00415\">10.1104/pp.16.00415</a>."},"department":[{"_id":"EvBe"}],"oa_version":"Published Version","status":"public","article_type":"original","issue":"2","publisher":"American Society of Plant Biologists","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"abstract":[{"text":"Cytokinin is a phytohormone that is well known for its roles in numerous plant growth and developmental processes, yet it has also been linked to abiotic stress response in a less defined manner. Arabidopsis (Arabidopsis thaliana) Cytokinin Response Factor 6 (CRF6) is a cytokinin-responsive AP2/ERF-family transcription factor that, through the cytokinin signaling pathway, plays a key role in the inhibition of dark-induced senescence. CRF6 expression is also induced by oxidative stress, and here we show a novel function for CRF6 in relation to oxidative stress and identify downstream transcriptional targets of CRF6 that are repressed in response to oxidative stress. Analysis of transcriptomic changes in wild-type and crf6 mutant plants treated with H2O2 identified CRF6-dependent differentially expressed transcripts, many of which were repressed rather than induced. Moreover, many repressed genes also show decreased expression in 35S:CRF6 overexpressing plants. Together, these findings suggest that CRF6 functions largely as a transcriptional repressor. Interestingly, among the H2O2 repressed CRF6-dependent transcripts was a set of five genes associated with cytokinin processes: (signaling) ARR6, ARR9, ARR11, (biosynthesis) LOG7, and (transport) ABCG14. We have examined mutants of these cytokinin-associated target genes to reveal novel connections to oxidative stress. Further examination of CRF6-DNA interactions indicated that CRF6 may regulate its targets both directly and indirectly. Together, this shows that CRF6 functions during oxidative stress as a negative regulator to control this cytokinin-associated module of CRF6- dependent genes and establishes a novel connection between cytokinin and oxidative stress response.","lang":"eng"}],"year":"2016","_id":"1331","volume":172,"author":[{"last_name":"Zwack","full_name":"Zwack, Paul","first_name":"Paul"},{"first_name":"Inge","full_name":"De Clercq, Inge","last_name":"De Clercq"},{"first_name":"Timothy","full_name":"Howton, Timothy","last_name":"Howton"},{"first_name":"H Tucker","last_name":"Hallmark","full_name":"Hallmark, H Tucker"},{"first_name":"Andrej","last_name":"Hurny","full_name":"Hurny, Andrej","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Erika","full_name":"Keshishian, Erika","last_name":"Keshishian"},{"last_name":"Parish","full_name":"Parish, Alyssa","first_name":"Alyssa"},{"full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"last_name":"Mukhtar","full_name":"Mukhtar, M Shahid","first_name":"M Shahid"},{"last_name":"Van Breusegem","full_name":"Van Breusegem, Frank","first_name":"Frank"},{"last_name":"Rashotte","full_name":"Rashotte, Aaron","first_name":"Aaron"}],"doi":"10.1104/pp.16.00415","day":"02","publication_status":"published","publication":"Plant Physiology","date_published":"2016-10-02T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"1249 - 1258","language":[{"iso":"eng"}],"publist_id":"5937","quality_controlled":"1","intvolume":"       172","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1104/pp.16.00415"}],"oa":1}