---
_id: '13355'
abstract:
- lang: eng
  text: 'Supramolecular self-assembly in biological systems holds promise to convert
    and amplify disease-specific signals to physical or mechanical signals that can
    direct cell fate. However, it remains challenging to design physiologically stable
    self-assembling systems that demonstrate tunable and predictable behavior. Here,
    the use of zwitterionic tetrapeptide modalities to direct nanoparticle assembly
    under physiological conditions is reported. The self-assembly of gold nanoparticles
    can be activated by enzymatic unveiling of surface-bound zwitterionic tetrapeptides
    through matrix metalloprotease-9 (MMP-9), which is overexpressed by cancer cells.
    This robust nanoparticle assembly is achieved by multivalent, self-complementary
    interactions of the zwitterionic tetrapeptides. In cancer cells that overexpress
    MMP-9, the nanoparticle assembly process occurs near the cell membrane and causes
    size-induced selection of cellular uptake mechanism, resulting in diminished cell
    growth. The enzyme responsiveness, and therefore, indirectly, the uptake route
    of the system can be programmed by customizing the peptide sequence: a simple
    inversion of the two amino acids at the cleavage site completely inactivates the
    enzyme responsiveness, self-assembly, and consequently changes the endocytic pathway.
    This robust self-complementary, zwitterionic peptide design demonstrates the use
    of enzyme-activated electrostatic side-chain patterns as powerful and customizable
    peptide modalities to program nanoparticle self-assembly and alter cellular response
    in biological context.'
article_number: '2104962'
article_processing_charge: No
article_type: original
author:
- first_name: Richard H.
  full_name: Huang, Richard H.
  last_name: Huang
- first_name: Nazia
  full_name: Nayeem, Nazia
  last_name: Nayeem
- first_name: Ye
  full_name: He, Ye
  last_name: He
- first_name: Jorge
  full_name: Morales, Jorge
  last_name: Morales
- first_name: Duncan
  full_name: Graham, Duncan
  last_name: Graham
- first_name: Rafal
  full_name: Klajn, Rafal
  id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b
  last_name: Klajn
- first_name: Maria
  full_name: Contel, Maria
  last_name: Contel
- first_name: Stephen
  full_name: O'Brien, Stephen
  last_name: O'Brien
- first_name: Rein V.
  full_name: Ulijn, Rein V.
  last_name: Ulijn
citation:
  ama: Huang RH, Nayeem N, He Y, et al. Self‐complementary zwitterionic peptides direct
    nanoparticle assembly and enable enzymatic selection of endocytic pathways. <i>Advanced
    Materials</i>. 2022;34(1). doi:<a href="https://doi.org/10.1002/adma.202104962">10.1002/adma.202104962</a>
  apa: Huang, R. H., Nayeem, N., He, Y., Morales, J., Graham, D., Klajn, R., … Ulijn,
    R. V. (2022). Self‐complementary zwitterionic peptides direct nanoparticle assembly
    and enable enzymatic selection of endocytic pathways. <i>Advanced Materials</i>.
    Wiley. <a href="https://doi.org/10.1002/adma.202104962">https://doi.org/10.1002/adma.202104962</a>
  chicago: Huang, Richard H., Nazia Nayeem, Ye He, Jorge Morales, Duncan Graham, Rafal
    Klajn, Maria Contel, Stephen O’Brien, and Rein V. Ulijn. “Self‐complementary Zwitterionic
    Peptides Direct Nanoparticle Assembly and Enable Enzymatic Selection of Endocytic
    Pathways.” <i>Advanced Materials</i>. Wiley, 2022. <a href="https://doi.org/10.1002/adma.202104962">https://doi.org/10.1002/adma.202104962</a>.
  ieee: R. H. Huang <i>et al.</i>, “Self‐complementary zwitterionic peptides direct
    nanoparticle assembly and enable enzymatic selection of endocytic pathways,” <i>Advanced
    Materials</i>, vol. 34, no. 1. Wiley, 2022.
  ista: Huang RH, Nayeem N, He Y, Morales J, Graham D, Klajn R, Contel M, O’Brien
    S, Ulijn RV. 2022. Self‐complementary zwitterionic peptides direct nanoparticle
    assembly and enable enzymatic selection of endocytic pathways. Advanced Materials.
    34(1), 2104962.
  mla: Huang, Richard H., et al. “Self‐complementary Zwitterionic Peptides Direct
    Nanoparticle Assembly and Enable Enzymatic Selection of Endocytic Pathways.” <i>Advanced
    Materials</i>, vol. 34, no. 1, 2104962, Wiley, 2022, doi:<a href="https://doi.org/10.1002/adma.202104962">10.1002/adma.202104962</a>.
  short: R.H. Huang, N. Nayeem, Y. He, J. Morales, D. Graham, R. Klajn, M. Contel,
    S. O’Brien, R.V. Ulijn, Advanced Materials 34 (2022).
date_created: 2023-08-01T09:33:26Z
date_published: 2022-01-06T00:00:00Z
date_updated: 2023-08-07T09:58:17Z
day: '06'
doi: 10.1002/adma.202104962
extern: '1'
external_id:
  pmid:
  - '34668253'
fulldoi: https://doi.org/10.1002/adma.202104962
intvolume: '        34'
issue: '1'
keyword:
- Mechanical Engineering
- Mechanics of Materials
- General Materials Science
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1002/adma.202104962
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Materials
publication_identifier:
  eissn:
  - 1521-4095
  issn:
  - 0935-9648
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Self‐complementary zwitterionic peptides direct nanoparticle assembly and enable
  enzymatic selection of endocytic pathways
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 34
year: '2022'
...