---
_id: '1342'
abstract:
- lang: eng
  text: A key aspect of bacterial survival is the ability to evolve while migrating
    across spatially varying environmental challenges. Laboratory experiments, however,
    often study evolution in well-mixed systems. Here, we introduce an experimental
    device, the microbial evolution and growth arena (MEGA)-plate, in which bacteria
    spread and evolved on a large antibiotic landscape (120 × 60 centimeters) that
    allowed visual observation of mutation and selection in a migrating bacterial
    front.While resistance increased consistently, multiple coexisting lineages diversified
    both phenotypically and genotypically. Analyzing mutants at and behind the propagating
    front,we found that evolution is not always led by the most resistant mutants;
    highly resistant mutants may be trapped behindmore sensitive lineages.TheMEGA-plate
    provides a versatile platformfor studying microbial adaption and directly visualizing
    evolutionary dynamics.
article_processing_charge: No
author:
- first_name: Michael
  full_name: Baym, Michael
  last_name: Baym
- first_name: Tami
  full_name: Lieberman, Tami
  last_name: Lieberman
- first_name: Eric
  full_name: Kelsic, Eric
  last_name: Kelsic
- first_name: Remy P
  full_name: Chait, Remy P
  id: 3464AE84-F248-11E8-B48F-1D18A9856A87
  last_name: Chait
  orcid: 0000-0003-0876-3187
- first_name: Rotem
  full_name: Gross, Rotem
  last_name: Gross
- first_name: Idan
  full_name: Yelin, Idan
  last_name: Yelin
- first_name: Roy
  full_name: Kishony, Roy
  last_name: Kishony
citation:
  ama: Baym M, Lieberman T, Kelsic E, et al. Spatiotemporal microbial evolution on
    antibiotic landscapes. <i>Science</i>. 2016;353(6304):1147-1151. doi:<a href="https://doi.org/10.1126/science.aag0822">10.1126/science.aag0822</a>
  apa: Baym, M., Lieberman, T., Kelsic, E., Chait, R. P., Gross, R., Yelin, I., &#38;
    Kishony, R. (2016). Spatiotemporal microbial evolution on antibiotic landscapes.
    <i>Science</i>. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.aag0822">https://doi.org/10.1126/science.aag0822</a>
  chicago: Baym, Michael, Tami Lieberman, Eric Kelsic, Remy P Chait, Rotem Gross,
    Idan Yelin, and Roy Kishony. “Spatiotemporal Microbial Evolution on Antibiotic
    Landscapes.” <i>Science</i>. American Association for the Advancement of Science,
    2016. <a href="https://doi.org/10.1126/science.aag0822">https://doi.org/10.1126/science.aag0822</a>.
  ieee: M. Baym <i>et al.</i>, “Spatiotemporal microbial evolution on antibiotic landscapes,”
    <i>Science</i>, vol. 353, no. 6304. American Association for the Advancement of
    Science, pp. 1147–1151, 2016.
  ista: Baym M, Lieberman T, Kelsic E, Chait RP, Gross R, Yelin I, Kishony R. 2016.
    Spatiotemporal microbial evolution on antibiotic landscapes. Science. 353(6304),
    1147–1151.
  mla: Baym, Michael, et al. “Spatiotemporal Microbial Evolution on Antibiotic Landscapes.”
    <i>Science</i>, vol. 353, no. 6304, American Association for the Advancement of
    Science, 2016, pp. 1147–51, doi:<a href="https://doi.org/10.1126/science.aag0822">10.1126/science.aag0822</a>.
  short: M. Baym, T. Lieberman, E. Kelsic, R.P. Chait, R. Gross, I. Yelin, R. Kishony,
    Science 353 (2016) 1147–1151.
date_created: 2018-12-11T11:51:29Z
date_published: 2016-09-09T00:00:00Z
date_updated: 2025-09-22T08:17:11Z
day: '09'
department:
- _id: CaGu
- _id: GaTk
doi: 10.1126/science.aag0822
external_id:
  isi:
  - '000382626800052'
intvolume: '       353'
isi: 1
issue: '6304'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534434/
month: '09'
oa: 1
oa_version: Preprint
page: 1147 - 1151
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5911'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Spatiotemporal microbial evolution on antibiotic landscapes
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 353
year: '2016'
...