---
_id: '1344'
abstract:
- lang: eng
  text: Despite being composed of immobile cells, plants reorient along directional
    stimuli. The hormone auxin is redistributed in stimulated organs leading to differential
    growth and bending. Auxin application triggers rapid cell wall acidification and
    elongation of aerial organs of plants, but the molecular players mediating these
    effects are still controversial. Here we use genetically-encoded pH and auxin
    signaling sensors, pharmacological and genetic manipulations available for Arabidopsis
    etiolated hypocotyls to clarify how auxin is perceived and the downstream growth
    executed. We show that auxin-induced acidification occurs by local activation
    of H+-ATPases, which in the context of gravity response is restricted to the lower
    organ side. This auxin-stimulated acidification and growth require TIR1/AFB-Aux/IAA
    nuclear auxin perception. In addition, auxin-induced gene transcription and specifically
    SAUR proteins are crucial downstream mediators of this growth. Our study provides
    strong experimental support for the acid growth theory and clarified the contribution
    of the upstream auxin perception mechanisms.
acknowledgement: "The authors express their gratitude to Veronika Bierbaum, Robert
  Hauschild for help with MATLAB,\r\nDaniel von Wangenheim for the gravitropism assay.
  We are thankful to Bill Gray, Mark Estelle,\r\nMichael Prigge, Ottoline Leyser,
  Claudia Oecking for sharing the seeds with us. We thank Katelyn\r\nSageman-Furnas
  and the members of the Friml lab for critical reading of the manuscript. The\r\nresearch
  leading to these results has received funding from the People Programme (Marie Curie\r\nActions)
  of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant\r\nagreement
  n° 291734. This work was also supported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP)."
article_number: e19048
article_processing_charge: No
author:
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Jeffrey
  full_name: Leung, Jeffrey
  last_name: Leung
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Fendrych M, Leung J, Friml J. TIR1 AFB Aux IAA auxin perception mediates rapid
    cell wall acidification and growth of Arabidopsis hypocotyls. <i>eLife</i>. 2016;5.
    doi:<a href="https://doi.org/10.7554/eLife.19048">10.7554/eLife.19048</a>
  apa: Fendrych, M., Leung, J., &#38; Friml, J. (2016). TIR1 AFB Aux IAA auxin perception
    mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. <i>ELife</i>.
    eLife Sciences Publications. <a href="https://doi.org/10.7554/eLife.19048">https://doi.org/10.7554/eLife.19048</a>
  chicago: Fendrych, Matyas, Jeffrey Leung, and Jiří Friml. “TIR1 AFB Aux IAA Auxin
    Perception Mediates Rapid Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.”
    <i>ELife</i>. eLife Sciences Publications, 2016. <a href="https://doi.org/10.7554/eLife.19048">https://doi.org/10.7554/eLife.19048</a>.
  ieee: M. Fendrych, J. Leung, and J. Friml, “TIR1 AFB Aux IAA auxin perception mediates
    rapid cell wall acidification and growth of Arabidopsis hypocotyls,” <i>eLife</i>,
    vol. 5. eLife Sciences Publications, 2016.
  ista: Fendrych M, Leung J, Friml J. 2016. TIR1 AFB Aux IAA auxin perception mediates
    rapid cell wall acidification and growth of Arabidopsis hypocotyls. eLife. 5,
    e19048.
  mla: Fendrych, Matyas, et al. “TIR1 AFB Aux IAA Auxin Perception Mediates Rapid
    Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.” <i>ELife</i>, vol.
    5, e19048, eLife Sciences Publications, 2016, doi:<a href="https://doi.org/10.7554/eLife.19048">10.7554/eLife.19048</a>.
  short: M. Fendrych, J. Leung, J. Friml, ELife 5 (2016).
corr_author: '1'
date_created: 2018-12-11T11:51:29Z
date_published: 2016-09-14T00:00:00Z
date_updated: 2025-09-22T08:16:03Z
day: '14'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.7554/eLife.19048
ec_funded: 1
external_id:
  isi:
  - '000385559100001'
file:
- access_level: open_access
  checksum: 9209541fbba00f24daad21a5d568540d
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:09:24Z
  date_updated: 2020-07-14T12:44:45Z
  file_id: '4748'
  file_name: IST-2016-693-v1+1_e19048-download.pdf
  file_size: 5666343
  relation: main_file
file_date_updated: 2020-07-14T12:44:45Z
fulldoi: https://doi.org/10.7554/eLife.19048
has_accepted_license: '1'
intvolume: '         5'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5908'
pubrep_id: '654'
quality_controlled: '1'
scopus_import: '1'
status: public
title: TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and
  growth of Arabidopsis hypocotyls
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 5
year: '2016'
...