{"supervisor":[{"last_name":"Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","year":"2016","date_created":"2018-12-11T11:51:46Z","publication_status":"published","publist_id":"5811","doi_confirm":"1","date_updated":"2026-07-29T11:31:52Z","date_published":"2016-03-01T00:00:00Z","department":[{"_id":"PeJo"},{"_id":"GradSch"}],"abstract":[{"text":"CA3 pyramidal neurons are thought to pay a key role in memory storage and pattern completion by activity-dependent synaptic plasticity between CA3-CA3 recurrent excitatory synapses. To examine the induction rules of synaptic plasticity at CA3-CA3 synapses, we performed whole-cell patch-clamp recordings in acute hippocampal slices from rats (postnatal 21-24 days) at room temperature. Compound excitatory postsynaptic potentials (ESPSs) were recorded by tract stimulation in stratum oriens in the presence of 10 µM gabazine. High-frequency stimulation (HFS) induced N-methyl-D-aspartate (NMDA) receptor-dependent long-term potentiation (LTP). Although LTP by HFS did not requier postsynaptic spikes, it was blocked by Na+-channel blockers suggesting that local active processes (e.g.) dendritic spikes) may contribute to LTP induction without requirement of a somatic action potential (AP). We next examined the properties of spike timing-dependent plasticity (STDP) at CA3-CA3 synapses. Unexpectedly, low-frequency pairing of EPSPs and backpropagated action potentialy (bAPs) induced LTP, independent of temporal order. The STDP curve was symmetric and broad, with a half-width of ~150 ms. Consistent with these specific STDP induction properties, post-presynaptic sequences led to a supralinear summation of spine [Ca2+] transients. Furthermore, in autoassociative network models, storage and recall was substantially more robust with symmetric than with asymmetric STDP rules. In conclusion, we found associative forms of LTP at CA3-CA3 recurrent collateral synapses with distinct induction rules. LTP induced by HFS may be associated with dendritic spikes. In contrast, low frequency pairing of pre- and postsynaptic activity induced LTP only if EPSP-AP were temporally very close. Together, these induction mechanisms of synaptiic plasticity may contribute to memory storage in the CA3-CA3 microcircuit at different ranges of activity.","lang":"eng"}],"_id":"1396","oa_version":"Published Version","alternative_title":["ISTA Thesis"],"title":"Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","OA_place":"publisher","has_accepted_license":"1","degree_awarded":"PhD","month":"03","day":"01","related_material":{"record":[{"status":"public","id":"1432","relation":"part_of_dissertation"}]},"status":"public","corr_author":"1","publication_identifier":{"issn":["2663-337X"]},"file":[{"file_size":2407572,"date_updated":"2020-07-14T12:44:48Z","file_name":"Thesis_Mishra_Rajiv (Final).pdf","creator":"dernst","file_id":"6782","checksum":"5a010a838faf040f7064f3cfb802f743","relation":"main_file","access_level":"closed","content_type":"application/pdf","date_created":"2019-08-09T12:14:46Z"},{"relation":"main_file","checksum":"81b26d9ede92c99f1d8cc6fa1d04cbbb","content_type":"application/pdf","date_created":"2021-02-22T11:48:44Z","access_level":"open_access","file_name":"2016_RajivMishra_Thesis.pdf","date_updated":"2021-02-22T11:48:44Z","file_size":2407572,"success":1,"file_id":"9183","creator":"dernst"}],"publisher":"Institute of Science and Technology Austria","ddc":["570"],"oa":1,"language":[{"iso":"eng"}],"page":"83","citation":{"ieee":"R. K. Mishra, “Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus,” Institute of Science and Technology Austria, 2016.","ama":"Mishra RK. Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus. 2016.","short":"R.K. Mishra, Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses in Hippocampus, Institute of Science and Technology Austria, 2016.","mla":"Mishra, Rajiv Kumar. <i>Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses in Hippocampus</i>. Institute of Science and Technology Austria, 2016.","ista":"Mishra RK. 2016. Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus. Institute of Science and Technology Austria.","apa":"Mishra, R. K. (2016). <i>Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus</i>. Institute of Science and Technology Austria.","chicago":"Mishra, Rajiv Kumar. “Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses in Hippocampus.” Institute of Science and Technology Austria, 2016."},"author":[{"last_name":"Mishra","full_name":"Mishra, Rajiv Kumar","id":"46CB58F2-F248-11E8-B48F-1D18A9856A87","first_name":"Rajiv Kumar"}],"file_date_updated":"2021-02-22T11:48:44Z","type":"dissertation"}