article published yes Roger Hasler author Marie Helene Steger-Polt author Ciril Reiner-Rozman author Stefan Fossati author Seungho Lee author BB243B88-D767-11E9-B658-BC13E66974250000-0002-6962-8598 Patrik Aspermair author Christoph Kleber author Maria Ibáñez author 43C61214-F248-11E8-B48F-1D18A9856A870000-0001-5013-2843 Jakub Dostalek author Wolfgang Knoll author MaIb department The use of multimodal readout mechanisms next to label-free real-time monitoring of biomolecular interactions can provide valuable insight into surface-based reaction mechanisms. To this end, the combination of an electrolyte-gated field-effect transistor (EG-FET) with a fiber optic-coupled surface plasmon resonance (FO-SPR) probe serving as gate electrode has been investigated to deconvolute surface mass and charge density variations associated to surface reactions. However, applying an electrochemical potential on such gold-coated FO-SPR gate electrodes can induce gradual morphological changes of the thin gold film, leading to an irreversible blue-shift of the SPR wavelength and a substantial signal drift. We show that mild annealing leads to optical and electronic signal stabilization (20-fold lower signal drift than as-sputtered fiber optic gates) and improved overall analytical performance characteristics. The thermal treatment prevents morphological changes of the thin gold-film occurring during operation, hence providing reliable and stable data immediately upon gate voltage application. Thus, the readout output of both transducing principles, the optical FO-SPR and electronic EG-FET, stays constant throughout the whole sensing time-window and the long-term effect of thermal treatment is also improved, providing stable signals even after 1 year of storage. Annealing should therefore be considered a necessary modification for applying fiber optic gate electrodes in real-time multimodal investigations of surface reactions at the solid-liquid interface. https://research-explorer.ista.ac.at/download/13968/13978/2023_FrontiersPhysics_Hasler.pdf application/pdfno Frontiers2023 eng 2296-424X 00103863640000110.3389/fphy.2023.1202132 11 https://research-explorer.ista.ac.at/record/19308 Hasler, Roger, et al. “Optical and Electronic Signal Stabilization of Plasmonic Fiber Optic Gate Electrodes: Towards Improved Real-Time Dual-Mode Biosensing.” <i>Frontiers in Physics</i>, vol. 11, 1202132, Frontiers, 2023, doi:<a href="https://doi.org/10.3389/fphy.2023.1202132">10.3389/fphy.2023.1202132</a>. Hasler, R., Steger-Polt, M. H., Reiner-Rozman, C., Fossati, S., Lee, S., Aspermair, P., … Knoll, W. (2023). Optical and electronic signal stabilization of plasmonic fiber optic gate electrodes: Towards improved real-time dual-mode biosensing. <i>Frontiers in Physics</i>. Frontiers. <a href="https://doi.org/10.3389/fphy.2023.1202132">https://doi.org/10.3389/fphy.2023.1202132</a> Hasler, Roger, Marie Helene Steger-Polt, Ciril Reiner-Rozman, Stefan Fossati, Seungho Lee, Patrik Aspermair, Christoph Kleber, Maria Ibáñez, Jakub Dostalek, and Wolfgang Knoll. “Optical and Electronic Signal Stabilization of Plasmonic Fiber Optic Gate Electrodes: Towards Improved Real-Time Dual-Mode Biosensing.” <i>Frontiers in Physics</i>. Frontiers, 2023. <a href="https://doi.org/10.3389/fphy.2023.1202132">https://doi.org/10.3389/fphy.2023.1202132</a>. Hasler R, Steger-Polt MH, Reiner-Rozman C, et al. Optical and electronic signal stabilization of plasmonic fiber optic gate electrodes: Towards improved real-time dual-mode biosensing. <i>Frontiers in Physics</i>. 2023;11. doi:<a href="https://doi.org/10.3389/fphy.2023.1202132">10.3389/fphy.2023.1202132</a> Hasler R, Steger-Polt MH, Reiner-Rozman C, Fossati S, Lee S, Aspermair P, Kleber C, Ibáñez M, Dostalek J, Knoll W. 2023. Optical and electronic signal stabilization of plasmonic fiber optic gate electrodes: Towards improved real-time dual-mode biosensing. Frontiers in Physics. 11, 1202132. R. Hasler <i>et al.</i>, “Optical and electronic signal stabilization of plasmonic fiber optic gate electrodes: Towards improved real-time dual-mode biosensing,” <i>Frontiers in Physics</i>, vol. 11. Frontiers, 2023. R. Hasler, M.H. Steger-Polt, C. Reiner-Rozman, S. Fossati, S. Lee, P. Aspermair, C. Kleber, M. Ibáñez, J. Dostalek, W. Knoll, Frontiers in Physics 11 (2023). 139682023-08-06T22:01:11Z2025-03-11T08:00:41Z