---
res:
  bibo_abstract:
  - Epithelial barrier function is commonly analyzed using transepithelial electrical
    resistance, which measures ion flux across a monolayer, or by adding traceable
    macromolecules and monitoring their passage across the monolayer. Although these
    methods measure changes in global barrier function, they lack the sensitivity
    needed to detect local or transient barrier breaches, and they do not reveal the
    location of barrier leaks. Therefore, we previously developed a method that we
    named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which
    overcomes these limitations, allowing for detection of local tight junction leaks
    with high spatiotemporal resolution. Here, we present expanded applications for
    ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier
    restoration and actin accumulation following laser injury. ZnUMBA can also be
    effectively utilized in developing zebrafish embryos as well as cultured monolayers
    of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful
    and flexible method that, with minimal optimization, can be applied to multiple
    systems to measure dynamic changes in barrier function with spatiotemporal precision.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Tomohito
      foaf_name: Higashi, Tomohito
      foaf_surname: Higashi
  - foaf_Person:
      foaf_givenName: Rachel E.
      foaf_name: Stephenson, Rachel E.
      foaf_surname: Stephenson
  - foaf_Person:
      foaf_givenName: Cornelia
      foaf_name: Schwayer, Cornelia
      foaf_surname: Schwayer
      foaf_workInfoHomepage: http://www.librecat.org/personId=3436488C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-5130-2226
  - foaf_Person:
      foaf_givenName: Karla
      foaf_name: Huljev, Karla
      foaf_surname: Huljev
      foaf_workInfoHomepage: http://www.librecat.org/personId=44C6F6A6-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Atsuko Y.
      foaf_name: Higashi, Atsuko Y.
      foaf_surname: Higashi
  - foaf_Person:
      foaf_givenName: Carl-Philipp J
      foaf_name: Heisenberg, Carl-Philipp J
      foaf_surname: Heisenberg
      foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-0912-4566
  - foaf_Person:
      foaf_givenName: Hideki
      foaf_name: Chiba, Hideki
      foaf_surname: Chiba
  - foaf_Person:
      foaf_givenName: Ann L.
      foaf_name: Miller, Ann L.
      foaf_surname: Miller
  bibo_doi: 10.1242/jcs.260668
  bibo_issue: '15'
  bibo_volume: 136
  dct_date: 2023^xs_gYear
  dct_identifier:
  - UT:001070149000001
  dct_isPartOf:
  - http://id.crossref.org/issn/0021-9533
  - http://id.crossref.org/issn/1477-9137
  dct_language: eng
  dct_publisher: The Company of Biologists@
  dct_title: ZnUMBA - a live imaging method to detect local barrier breaches@
  fabio_hasPubmedId: '37461809'
...