{"publication":"Journal of Cell Science","issue":"15","publication_status":"published","month":"08","date_created":"2023-08-20T22:01:13Z","external_id":{"isi":["001070149000001"]},"publisher":"The Company of Biologists","article_type":"original","status":"public","_id":"14082","file":[{"date_updated":"2023-08-21T07:37:54Z","access_level":"closed","creator":"dernst","file_size":18665315,"embargo":"2024-08-10","date_created":"2023-08-21T07:37:54Z","file_name":"2023_JourCellScience_Higashi.pdf","checksum":"a399389b7e3d072f1788b63e612a10b3","embargo_to":"open_access","content_type":"application/pdf","file_id":"14092","relation":"main_file"}],"acknowledgement":"The authors thank their respective lab members for feedback and helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria for their support.\r\nThis work was supported by the National Institutes of Health [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for Biomedical Engineering Research from the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the European Research Council [advanced grant 742573 to C.-P.H.]. ","ec_funded":1,"file_date_updated":"2023-08-21T07:37:54Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","date_updated":"2023-12-13T12:11:18Z","department":[{"_id":"CaHe"},{"_id":"EvBe"}],"oa_version":"None","doi":"10.1242/jcs.260668","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020"}],"day":"01","language":[{"iso":"eng"}],"author":[{"last_name":"Higashi","first_name":"Tomohito","full_name":"Higashi, Tomohito"},{"first_name":"Rachel E.","last_name":"Stephenson","full_name":"Stephenson, Rachel E."},{"full_name":"Schwayer, Cornelia","id":"3436488C-F248-11E8-B48F-1D18A9856A87","first_name":"Cornelia","orcid":"0000-0001-5130-2226","last_name":"Schwayer"},{"full_name":"Huljev, Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","first_name":"Karla","last_name":"Huljev"},{"full_name":"Higashi, Atsuko Y.","last_name":"Higashi","first_name":"Atsuko Y."},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"full_name":"Chiba, Hideki","first_name":"Hideki","last_name":"Chiba"},{"full_name":"Miller, Ann L.","last_name":"Miller","first_name":"Ann L."}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"isi":1,"year":"2023","abstract":[{"text":"Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision.","lang":"eng"}],"quality_controlled":"1","date_published":"2023-08-01T00:00:00Z","volume":136,"scopus_import":"1","ddc":["570"],"publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"article_processing_charge":"No","citation":{"ista":"Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ, Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. 136(15), jcs260668.","mla":"Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>, vol. 136, no. 15, jcs260668, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>.","short":"T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J. Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).","apa":"Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg, C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>","ama":"Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. 2023;136(15). doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>","chicago":"Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev, Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>.","ieee":"T. Higashi <i>et al.</i>, “ZnUMBA - a live imaging method to detect local barrier breaches,” <i>Journal of Cell Science</i>, vol. 136, no. 15. The Company of Biologists, 2023."},"intvolume":"       136","title":"ZnUMBA - a live imaging method to detect local barrier breaches","article_number":"jcs260668","has_accepted_license":"1"}