---
OA_place: publisher
OA_type: free access
_id: '14082'
abstract:
- lang: eng
  text: Epithelial barrier function is commonly analyzed using transepithelial electrical
    resistance, which measures ion flux across a monolayer, or by adding traceable
    macromolecules and monitoring their passage across the monolayer. Although these
    methods measure changes in global barrier function, they lack the sensitivity
    needed to detect local or transient barrier breaches, and they do not reveal the
    location of barrier leaks. Therefore, we previously developed a method that we
    named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which
    overcomes these limitations, allowing for detection of local tight junction leaks
    with high spatiotemporal resolution. Here, we present expanded applications for
    ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier
    restoration and actin accumulation following laser injury. ZnUMBA can also be
    effectively utilized in developing zebrafish embryos as well as cultured monolayers
    of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful
    and flexible method that, with minimal optimization, can be applied to multiple
    systems to measure dynamic changes in barrier function with spatiotemporal precision.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "The authors thank their respective lab members for feedback and
  helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria
  for their support.\r\nThis work was supported by the National Institutes of Health
  [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan
  Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for
  Biomedical Engineering Research from the Nakatani Foundation for Advancement of
  Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the
  European Research Council [advanced grant 742573 to C.-P.H.]. "
article_number: jcs260668
article_processing_charge: No
article_type: original
author:
- first_name: Tomohito
  full_name: Higashi, Tomohito
  last_name: Higashi
- first_name: Rachel E.
  full_name: Stephenson, Rachel E.
  last_name: Stephenson
- first_name: Cornelia
  full_name: Schwayer, Cornelia
  id: 3436488C-F248-11E8-B48F-1D18A9856A87
  last_name: Schwayer
  orcid: 0000-0001-5130-2226
- first_name: Karla
  full_name: Huljev, Karla
  id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
  last_name: Huljev
- first_name: Atsuko Y.
  full_name: Higashi, Atsuko Y.
  last_name: Higashi
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Hideki
  full_name: Chiba, Hideki
  last_name: Chiba
- first_name: Ann L.
  full_name: Miller, Ann L.
  last_name: Miller
citation:
  ama: Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method
    to detect local barrier breaches. <i>Journal of Cell Science</i>. 2023;136(15).
    doi:<a href="https://doi.org/10.1242/jcs.260668">10.1242/jcs.260668</a>
  apa: Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg,
    C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local
    barrier breaches. <i>Journal of Cell Science</i>. The Company of Biologists. <a
    href="https://doi.org/10.1242/jcs.260668">https://doi.org/10.1242/jcs.260668</a>
  chicago: Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev,
    Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller.
    “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal
    of Cell Science</i>. The Company of Biologists, 2023. <a href="https://doi.org/10.1242/jcs.260668">https://doi.org/10.1242/jcs.260668</a>.
  ieee: T. Higashi <i>et al.</i>, “ZnUMBA - a live imaging method to detect local
    barrier breaches,” <i>Journal of Cell Science</i>, vol. 136, no. 15. The Company
    of Biologists, 2023.
  ista: Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ,
    Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier
    breaches. Journal of Cell Science. 136(15), jcs260668.
  mla: Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier
    Breaches.” <i>Journal of Cell Science</i>, vol. 136, no. 15, jcs260668, The Company
    of Biologists, 2023, doi:<a href="https://doi.org/10.1242/jcs.260668">10.1242/jcs.260668</a>.
  short: T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J.
    Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).
date_created: 2023-08-20T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2025-06-25T06:28:45Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: EvBe
doi: 10.1242/jcs.260668
ec_funded: 1
external_id:
  isi:
  - '001070149000001'
  pmid:
  - '37461809'
has_accepted_license: '1'
intvolume: '       136'
isi: 1
issue: '15'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1242/jcs.260668
month: '08'
oa: 1
oa_version: None
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Journal of Cell Science
publication_identifier:
  eissn:
  - 1477-9137
  issn:
  - 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: ZnUMBA - a live imaging method to detect local barrier breaches
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 136
year: '2023'
...