---
res:
  bibo_abstract:
  - 'The bacteriophage M13 has found frequent applications in nanobiotechnology due
    to its chemically and genetically tunable protein surface and its ability to self-assemble
    into colloidal membranes. Additionally, its single-stranded (ss) genome is commonly
    used as scaffold for DNA origami. Despite the manifold uses of M13, upstream production
    methods for phage and scaffold ssDNA are underexamined with respect to future
    industrial usage. Here, the high-cell-density phage production with Escherichia
    coli as host organism was studied in respect of medium composition, infection
    time, multiplicity of infection, and specific growth rate. The specific growth
    rate and the multiplicity of infection were identified as the crucial state variables
    that influence phage amplification rate on one hand and the concentration of produced
    ssDNA on the other hand. Using a growth rate of 0.15 h−1 and a multiplicity of
    infection of 0.05 pfu cfu−1 in the fed-batch production process, the concentration
    of pure isolated M13 ssDNA usable for scaffolded DNA origami could be enhanced
    by 54% to 590 mg L−1. Thus, our results help enabling M13 production for industrial
    uses in nanobiotechnology. Biotechnol. Bioeng. 2017;114: 777–784.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Benjamin
      foaf_name: Kick, Benjamin
      foaf_surname: Kick
  - foaf_Person:
      foaf_givenName: Samantha
      foaf_name: Hensler, Samantha
      foaf_surname: Hensler
  - foaf_Person:
      foaf_givenName: Florian M
      foaf_name: Praetorius, Florian M
      foaf_surname: Praetorius
      foaf_workInfoHomepage: http://www.librecat.org/personId=dfec9381-4341-11ee-8fd8-faa02bba7d62
  - foaf_Person:
      foaf_givenName: Hendrik
      foaf_name: Dietz, Hendrik
      foaf_surname: Dietz
  - foaf_Person:
      foaf_givenName: Dirk
      foaf_name: Weuster-Botz, Dirk
      foaf_surname: Weuster-Botz
  bibo_doi: 10.1002/bit.26200
  bibo_issue: '4'
  bibo_volume: 114
  dct_date: 2017^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0006-3592
  dct_language: eng
  dct_publisher: Wiley@
  dct_subject:
  - Applied Microbiology and Biotechnology
  - Bioengineering
  - Biotechnology
  dct_title: Specific growth rate and multiplicity of infection affect high-cell-density
    fermentation with bacteriophage M13 for ssDNA production@
  fabio_hasPubmedId: '27748519'
...