{"date_created":"2023-09-06T12:51:14Z","month":"11","publication_status":"published","publisher":"Wiley","external_id":{"pmid":["25346175"]},"publication":"Angewandte Chemie International Edition","issue":"47","doi":"10.1002/ange.201405991","oa_version":"Published Version","type":"journal_article","date_updated":"2023-11-07T12:14:30Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","article_type":"original","extern":"1","_id":"14301","abstract":[{"text":"DNA has become a prime material for assembling complex three-dimensional objects that promise utility in various areas of application. However, achieving user-defined goals with DNA objects has been hampered by the difficulty to prepare them at arbitrary concentrations and in user-defined solution conditions. Here, we describe a method that solves this problem. The method is based on poly(ethylene glycol)-induced depletion of species with high molecular weight. We demonstrate that our method is applicable to a wide spectrum of DNA shapes and that it achieves excellent recovery yields of target objects up to 97 %, while providing efficient separation from non-integrated DNA strands. DNA objects may be prepared at concentrations up to the limit of solubility, including the possibility for bringing DNA objects into a solid phase. Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects.","lang":"eng"}],"oa":1,"year":"2014","author":[{"full_name":"Stahl, Evi","first_name":"Evi","last_name":"Stahl"},{"full_name":"Martin, Thomas","last_name":"Martin","first_name":"Thomas"},{"id":"dfec9381-4341-11ee-8fd8-faa02bba7d62","full_name":"Praetorius, Florian M","last_name":"Praetorius","first_name":"Florian M"},{"full_name":"Dietz, Hendrik","first_name":"Hendrik","last_name":"Dietz"}],"language":[{"iso":"eng"}],"day":"17","main_file_link":[{"url":"https://doi.org/10.1002/ange.201405991","open_access":"1"}],"page":"12949-12954","citation":{"ieee":"E. Stahl, T. Martin, F. M. Praetorius, and H. Dietz, “Facile and scalable preparation of pure and dense DNA origami solutions,” <i>Angewandte Chemie International Edition</i>, vol. 126, no. 47. Wiley, pp. 12949–12954, 2014.","short":"E. Stahl, T. Martin, F.M. Praetorius, H. Dietz, Angewandte Chemie International Edition 126 (2014) 12949–12954.","apa":"Stahl, E., Martin, T., Praetorius, F. M., &#38; Dietz, H. (2014). Facile and scalable preparation of pure and dense DNA origami solutions. <i>Angewandte Chemie International Edition</i>. Wiley. <a href=\"https://doi.org/10.1002/ange.201405991\">https://doi.org/10.1002/ange.201405991</a>","ama":"Stahl E, Martin T, Praetorius FM, Dietz H. Facile and scalable preparation of pure and dense DNA origami solutions. <i>Angewandte Chemie International Edition</i>. 2014;126(47):12949-12954. doi:<a href=\"https://doi.org/10.1002/ange.201405991\">10.1002/ange.201405991</a>","chicago":"Stahl, Evi, Thomas Martin, Florian M Praetorius, and Hendrik Dietz. “Facile and Scalable Preparation of Pure and Dense DNA Origami Solutions.” <i>Angewandte Chemie International Edition</i>. Wiley, 2014. <a href=\"https://doi.org/10.1002/ange.201405991\">https://doi.org/10.1002/ange.201405991</a>.","mla":"Stahl, Evi, et al. “Facile and Scalable Preparation of Pure and Dense DNA Origami Solutions.” <i>Angewandte Chemie International Edition</i>, vol. 126, no. 47, Wiley, 2014, pp. 12949–54, doi:<a href=\"https://doi.org/10.1002/ange.201405991\">10.1002/ange.201405991</a>.","ista":"Stahl E, Martin T, Praetorius FM, Dietz H. 2014. Facile and scalable preparation of pure and dense DNA origami solutions. Angewandte Chemie International Edition. 126(47), 12949–12954."},"title":"Facile and scalable preparation of pure and dense DNA origami solutions","intvolume":"       126","article_processing_charge":"No","publication_identifier":{"eissn":["1521-3773"],"issn":["1433-7851"]},"date_published":"2014-11-17T00:00:00Z","volume":126,"quality_controlled":"1","pmid":1,"scopus_import":"1"}