{"status":"public","abstract":[{"text":"Scaffolded DNA origami enables the fabrication of a variety of complex nanostructures that promise utility in diverse fields of application, ranging from biosensing over advanced therapeutics to metamaterials. The broad applicability of DNA origami as a material beyond the level of proof-of-concept studies critically depends, among other factors, on the availability of large amounts of pure single-stranded scaffold DNA. Here, we present a method for the efficient production of M13 bacteriophage-derived genomic DNA using high-cell-density fermentation of Escherichia coli in stirred-tank bioreactors. We achieve phage titers of up to 1.6 × 1014 plaque-forming units per mL. Downstream processing yields up to 410 mg of high-quality single-stranded DNA per one liter reaction volume, thus upgrading DNA origami-based nanotechnology from the milligram to the gram scale.","lang":"eng"}],"date_updated":"2023-11-07T11:56:32Z","intvolume":"        15","quality_controlled":"1","day":"01","extern":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1021/acs.nanolett.5b01461"}],"pmid":1,"volume":15,"external_id":{"pmid":["26028443"]},"article_type":"letter_note","date_created":"2023-09-06T12:52:47Z","publication":"Nano Letters","date_published":"2015-06-01T00:00:00Z","article_processing_charge":"No","year":"2015","author":[{"last_name":"Kick","first_name":"B","full_name":"Kick, B"},{"last_name":"Praetorius","id":"dfec9381-4341-11ee-8fd8-faa02bba7d62","first_name":"Florian M","full_name":"Praetorius, Florian M"},{"full_name":"Dietz, H","first_name":"H","last_name":"Dietz"},{"first_name":"D","full_name":"Weuster-Botz, D","last_name":"Weuster-Botz"}],"language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"4672-4676","oa":1,"publisher":"ACS Publications","publication_status":"published","month":"06","issue":"7","title":"Efficient production of single-stranded phage DNA as scaffolds for DNA origami","doi":"10.1021/acs.nanolett.5b01461","publication_identifier":{"eissn":["1530-6992"],"issn":["1530-6984"]},"_id":"14303","citation":{"apa":"Kick, B., Praetorius, F. M., Dietz, H., &#38; Weuster-Botz, D. (2015). Efficient production of single-stranded phage DNA as scaffolds for DNA origami. <i>Nano Letters</i>. ACS Publications. <a href=\"https://doi.org/10.1021/acs.nanolett.5b01461\">https://doi.org/10.1021/acs.nanolett.5b01461</a>","short":"B. Kick, F.M. Praetorius, H. Dietz, D. Weuster-Botz, Nano Letters 15 (2015) 4672–4676.","chicago":"Kick, B, Florian M Praetorius, H Dietz, and D Weuster-Botz. “Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami.” <i>Nano Letters</i>. ACS Publications, 2015. <a href=\"https://doi.org/10.1021/acs.nanolett.5b01461\">https://doi.org/10.1021/acs.nanolett.5b01461</a>.","ieee":"B. Kick, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Efficient production of single-stranded phage DNA as scaffolds for DNA origami,” <i>Nano Letters</i>, vol. 15, no. 7. ACS Publications, pp. 4672–4676, 2015.","mla":"Kick, B., et al. “Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami.” <i>Nano Letters</i>, vol. 15, no. 7, ACS Publications, 2015, pp. 4672–76, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.5b01461\">10.1021/acs.nanolett.5b01461</a>.","ama":"Kick B, Praetorius FM, Dietz H, Weuster-Botz D. Efficient production of single-stranded phage DNA as scaffolds for DNA origami. <i>Nano Letters</i>. 2015;15(7):4672-4676. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.5b01461\">10.1021/acs.nanolett.5b01461</a>","ista":"Kick B, Praetorius FM, Dietz H, Weuster-Botz D. 2015. Efficient production of single-stranded phage DNA as scaffolds for DNA origami. Nano Letters. 15(7), 4672–4676."},"type":"journal_article","oa_version":"Published Version"}