---
_id: '14562'
abstract:
- lang: eng
  text: "Regulation of the Arp2/3 complex is required for productive nucleation of
    branched actin networks. An emerging aspect of regulation is the incorporation
    of subunit isoforms into the Arp2/3 complex. Specifically, both ArpC5 subunit
    isoforms, ArpC5 and ArpC5L, have been reported to fine-tune nucleation activity
    and branch junction stability. We have combined reverse genetics and cellular
    structural biology to describe how ArpC5 and ArpC5L differentially affect cell
    migration. Both define the structural stability of ArpC1 in branch junctions and,
    in turn, by determining protrusion characteristics, affect protein dynamics and
    actin network ultrastructure. ArpC5 isoforms also affect the positioning of members
    of the Ena/Vasodilator-stimulated phosphoprotein (VASP) family of actin filament
    elongators, which mediate ArpC5 isoform–specific effects on the actin assembly
    level. Our results suggest that ArpC5 and Ena/VASP proteins are part of a signaling
    pathway enhancing cell migration.\r\n"
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: ScienComp
- _id: EM-Fac
acknowledgement: "We would like to thank K. von Peinen and B. Denker (Helmholtz Centre
  for Infection Research, Braunschweig, Germany) for experimental and technical assistance,
  respectively.\r\nFunding: This research was supported by the Scientific Service
  Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp),
  the Life Science Facility (LSF), the Imaging and Optics facility (IOF), and the
  Electron Microscopy Facility (EMF). We acknowledge support from ISTA and from the
  Austrian Science Fund (FWF) (P33367) to F.K.M.S., from the Research Training Group
  GRK2223 and the Helmholtz Society to K.R,. and from the Deutsche Forschungsgemeinschaft
  (DFG) to J.F. and K.R."
article_processing_charge: No
author:
- first_name: Florian KM
  full_name: Schur, Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
citation:
  ama: Schur FK. Research data of the publication “ArpC5 isoforms regulate Arp2/3
    complex-dependent protrusion through differential Ena/VASP positioning.” 2023.
    doi:<a href="https://doi.org/10.15479/AT:ISTA:14562">10.15479/AT:ISTA:14562</a>
  apa: Schur, F. K. (2023). Research data of the publication “ArpC5 isoforms regulate
    Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.”
    Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:14562">https://doi.org/10.15479/AT:ISTA:14562</a>
  chicago: Schur, Florian KM. “Research Data of the Publication ‘ArpC5 Isoforms Regulate
    Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.’”
    Institute of Science and Technology Austria, 2023. <a href="https://doi.org/10.15479/AT:ISTA:14562">https://doi.org/10.15479/AT:ISTA:14562</a>.
  ieee: F. K. Schur, “Research data of the publication ‘ArpC5 isoforms regulate Arp2/3
    complex-dependent protrusion through differential Ena/VASP positioning.’” Institute
    of Science and Technology Austria, 2023.
  ista: Schur FK. 2023. Research data of the publication ‘ArpC5 isoforms regulate
    Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning’,
    Institute of Science and Technology Austria, <a href="https://doi.org/10.15479/AT:ISTA:14562">10.15479/AT:ISTA:14562</a>.
  mla: Schur, Florian KM. <i>Research Data of the Publication “ArpC5 Isoforms Regulate
    Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.”</i>
    Institute of Science and Technology Austria, 2023, doi:<a href="https://doi.org/10.15479/AT:ISTA:14562">10.15479/AT:ISTA:14562</a>.
  short: F.K. Schur, (2023).
contributor:
- contributor_type: researcher
  first_name: Florian
  id: 404F5528-F248-11E8-B48F-1D18A9856A87
  last_name: Fäßler
  orcid: 0000-0001-7149-769X
- contributor_type: researcher
  first_name: Manjunath
  id: 305ab18b-dc7d-11ea-9b2f-b58195228ea2
  last_name: Javoor
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  first_name: Julia
  id: 3B12E2E6-F248-11E8-B48F-1D18A9856A87
  last_name: Datler
  orcid: 0000-0002-3616-8580
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  first_name: Hermann
  last_name: Döring
- contributor_type: researcher
  first_name: Florian
  id: b9d234ba-9e33-11ed-95b6-cd561df280e6
  last_name: Hofer
- contributor_type: researcher
  first_name: Georgi A
  id: 38C393BE-F248-11E8-B48F-1D18A9856A87
  last_name: Dimchev
  orcid: 0000-0001-8370-6161
- contributor_type: researcher
  first_name: Victor-Valentin
  id: 3661B498-F248-11E8-B48F-1D18A9856A87
  last_name: Hodirnau
- contributor_type: researcher
  first_name: Jan
  last_name: Faix
- contributor_type: researcher
  first_name: Klemens
  last_name: Rottner
- contributor_type: researcher
  first_name: Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
corr_author: '1'
date_created: 2023-11-20T09:22:33Z
date_published: 2023-11-21T00:00:00Z
date_updated: 2025-04-23T08:46:21Z
day: '21'
ddc:
- '570'
department:
- _id: FlSc
doi: 10.15479/AT:ISTA:14562
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has_accepted_license: '1'
license: https://creativecommons.org/licenses/by-sa/4.0/
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 9B954C5C-BA93-11EA-9121-9846C619BF3A
  grant_number: P33367
  name: Structure and isoform diversity of the Arp2/3 complex
publisher: Institute of Science and Technology Austria
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status: public
title: Research data of the publication "ArpC5 isoforms regulate Arp2/3 complex-dependent
  protrusion through differential Ena/VASP positioning"
tmp:
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type: research_data
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...