thesis published Mike Hennessey-Wesen author 3F338C72-F248-11E8-B48F-1D18A9856A87 Björn Hof supervisor GradSch department BjHo department International IST Doctoral Program project Mutation rates represent the net result of complex interactions among various cellular processes and can dramatically influence the evolutionary fate of microbial populations. However, many popular techniques used to study mutations are subject to the confounding effects of heredity and the subtleties of adaptation to selection, all of which make it difficult to observe any dynamic responses of mutation rates to fitness challenges. Furthermore, in spite of the ubiquity of quorum sensing systems across the bacterial domain and relevance for many physiological behaviors, the effects of such mechanisms on mutation rate and adaptation remain poorly understood. In the following work, I present the development of a microfluidic droplet-based method to measure single base-pair mutation rates in growing populations of the bacterium Escherichia coli. I use this method to observe a stress-induced increase in mutation rate that is mediated by luxS, a highly conserved bacterial quorum sensing component. I also show that the aforementioned increase in mutation rate, and its associated control by luxS, corresponds to a higher degree of adaptability under competitive environments. https://research-explorer.ista.ac.at/download/14641/14648/mike_thesis_v06-12-2023.odt application/vnd.oasis.opendocument.text https://research-explorer.ista.ac.at/download/14641/14649/mike_thesis_v06-12-2023.pdf application/pdf2026-11-18 https://research-explorer.ista.ac.at/download/14641/19720/2023_Hennessey_Michael_Thesis_print.pdf application/pdf Institute of Science and Technology Austria2023 eng microfluidicsmiceobiologymutationsquorum sensing 2663-337X10.15479/at:ista:14641 104 Hennessey-Wesen, M. (2023). <i>Adaptive mutation in E. coli modulated by luxS</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/at:ista:14641">https://doi.org/10.15479/at:ista:14641</a> M. Hennessey-Wesen, “Adaptive mutation in E. coli modulated by luxS,” Institute of Science and Technology Austria, 2023. Hennessey-Wesen M. 2023. Adaptive mutation in E. coli modulated by luxS. Institute of Science and Technology Austria. Hennessey-Wesen, Mike. <i>Adaptive Mutation in E. Coli Modulated by LuxS</i>. Institute of Science and Technology Austria, 2023, doi:<a href="https://doi.org/10.15479/at:ista:14641">10.15479/at:ista:14641</a>. Hennessey-Wesen, Mike. “Adaptive Mutation in E. Coli Modulated by LuxS.” Institute of Science and Technology Austria, 2023. <a href="https://doi.org/10.15479/at:ista:14641">https://doi.org/10.15479/at:ista:14641</a>. Hennessey-Wesen M. Adaptive mutation in E. coli modulated by luxS. 2023. doi:<a href="https://doi.org/10.15479/at:ista:14641">10.15479/at:ista:14641</a> M. Hennessey-Wesen, Adaptive Mutation in E. Coli Modulated by LuxS, Institute of Science and Technology Austria, 2023. 146412023-12-04T13:17:37Z2026-07-13T10:55:56Z